BaTiO3 nanocrystals tracking in Deep biological tissues using Adaptive optics: in-vivo investigations of intraneuronal transport abnormalities – BADASS

BADASS project focuses on the tracking of moving nanoparticles in cells and in-vivo, in both real-time and super-localization regimes. Implementing an optimized and fast adaptive optics loop to a two-photon tracking microscope (already developed) is at the technical heart of the project. The device aims to measure nanoparticle positions in depth with better than 3 nanometers accuracy and on millisecond time scales, which are the typical spatiotemporal scales of the dynamics of individual molecular motors. The system will be tested and improved by measuring intraneuronal transport in zebrafish larvae. In a second step, the larvae will be subjected an oxidative stress mimicking neuronal dysfunctions of a neurodegenerative disease to compare different biological conditions. In parallel, a fine analysis of the results by an unsupervised Bayesian approach will allow us to infer the parameters of the intraneuronal transport carefully and to compare the biological conditions rigorously.