Two-Photon Polymerization of Ruthenium-based Materials: a Microfabrication Platform for Electrochemiluminescent Multiplexing Sensors. – 2PhotonECL

The proposed project aims in elaborating an original ‘one pot’ two-photon polymerisation process for the photostructuration of Ruthenium-based materials devoted to the fabrication of electrochemilumiscent (ECL) multiplexing immunosensors at µm scale. This original approach takes benefit of recent technological developments in the domain of multiabsorption fabrication in order to generate periodic patterns of ECL active materials. A ruthenium-based two-photon activable photoinitiator should locally promote free radical polymerisation with the concomitant integration of its electro active part into the native polymer 3D network. We propose to take advantage of this novel µm-fabrication strategy for the development of fully-organic ECL-based immunosensors that imply: i) bioactive hydrogels whose mechanical response to a targeted antibody will promote a large amplitude ECL transduction. ii) a shape-encoded recognition approach allowing multiplexed detection on the basis of ECL imaging.