LightRIM : superresolved low cost Random Illumination Microscopy – LightRIM

Despite their high price, super-resolved fluorescence microscopes, either scanning or structured illumination microscopes (SIM), used in biology imaging platforms, often show degraded performances due to sample induced optical aberrations.

We have recently developed an easy to use techniquethat provides the same resolution as SIM while being robust to aberrations . The Random Illumination Microscope (RIM) is based on the use of random dynamic illumination and statistical data processing. RIM has demonstrated its ability to image at high resolution samples that were inaccessible to current super-resolution techniques [Mangeat2021].

The objective of this project is to realize a fibered dynamic random illumination module that can be adapted to all microscopes. By accompanying this module with an adapted data processing algorithm, super-resolved microscopy will become accessible to all biology laboratories at a lower cost (less than 20 000 euros).

This project is a partnership between the Laboratoire de biologie Intégrative, the Fresnel Institute and the French SME Oxxius specialized in the realization of laser sources in health biology.