IgH enhencers influence B lymphocyte nuclear organization and genome stability – 3D-Nucleus

The genome is divided into several compartments: the nucleosome, supranucleosome and nucleus. Gene position is precise and dynamic in the nucleus; genes can interact with each other via their enhancer and promoter regions. The immunoglobulin heavy chain locus harbors two enhancers: 3'RR and Eµ-MAR. We will study the role of these enhancers on nuclear territory dynamics and how they maintain the genomic integrity of B lymphocytes. Nuclear positioning of immunoglobulin loci, chromatin status, 3D folding, and B-cell genome integrity will be examined in murine models by partial or total deletion for these two enhancers. This project will elucidate the mechanisms that govern the dynamics of territories and this project will contribute to our understanding of the mechanisms that govern the balance between legitimate and illegitimate interactions.