Muscle release Extracellular vesicles in the context of Insulin-resistance associated with Diabetes (MEXID) – MEXID

Cells release information (RNA / Protein / Lipids) into lipid nanovesicles called extracellular vesicles (EVs). We have shown that EVs released by skeletal muscle during the development of insulin resistance had virus-like properties and transferred deleterious information between insulin-sensitive cells. However, the systemic role of EVs released by skeletal muscle has hitherto remained confined to the extrapolation of the results obtained in vitro due to the lack of animal models making it possible to follow their secretion / fate / integration in vivo. This project proposes to use zebrafish to bridge this scientific barrier. This model will express fluorescent proteins involved in the formation of EVs allowing them to be tracked from their secretion to their final destination in order to specify their target tissues and their biological functions, and the impact of obesity on their trafficking and their properties at whole body level.