Steroidogenomics analysis of adrenal steroid excess – STEROMICS

We will combine high-throughput approaches from genomics and steroid mass spectrometry studies to establish profiles of molecular abnormalities associated with steroid profiles in different adrenal pathologies. The studies will be carried out in parallel with the different «omics« on the same tissue fragments of adenoma and adrenal hyperplasia, representing a
wide spectrum of steroidogenesis abnormalities. The results of these high-throughput analyzes will be integrated by bioinformatics analysis
to identify the genetic and epigenetic determinants of steroidogenesis abnormalities.

At the 18-month stage of the project, we characterized molecular classes of benign adrenal tumors correlating with disruption of steroid secretion.

This will allow progress in the pathophysiology of steroid disorders observed in adrenal diseases and open perspectives in many non-adrenal metabolic or cardiovascular diseases where adrenal steroid abnormalities have been observed.

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Adrenal steroids control many physiological process and are altered in many diseases. Adrenal diseases causing steroid excess are
good models to study steroids synthesis and its dysregulation. Our aim is to understand the molecular mechanisms of adrenal
steroid dysregulation by combining various high-throughput genomics and steroidomics (mass spectrometry analysis) approaches.
We will use macronodular- and micronodular- adrenal hyperplasia as well as adrenal adenomas as models of a broad range of
steroids dysregulation. We will study the genomics (transcriptome, miRnome, methylome, snp, ngs), steroidomics (targeted and
non-targeted), and perform functional studies of steroids secretion in parallel on the same series of adrenal tissues. The integration
of theses various “omics” will allow to identify specific molecular and steroid profiles of steroid dysregulation. This will open new
perspectives to progress in the pathophysiology of various diseases associated with steroid exces