DS03 - Stimuler le renouveau industriel

Bacterial-based production of Genetically Encoded Responsive Capsules – GenCaps

Submission summary

Chemical bioengineers develop nowadays eco-friendly processes for the synthesis of high-value molecules, typically based on bacterial fermentations. This principle can be pushed forward by rerouting biosynthetic pathways to the control of in cellulo nano-assemblies and manufacture of fragile functional nano-objects. As a first proof of concept, GenCaps proposes to engineer the formulation of temperature-responsive lipid capsules in genetically modified bacteria. Objectives are 1) overexpression of lipids, under the control of recombinant membrane proteins "scaffolds", to form proteoliposomes densely covered by diverse peptide tags of interest, 2) versatile loading of the lipid capsules in cellulo (e.g. with overexpressed proteins/peptides), and 3) introduction of temperature-responsive chains on the outer corona to control release/targeting. The final goal is a fully encoded system of production, including fluorescent labeling and T-responsive (elastin-like) sequences. The post-chemical coupling of the capsules with fluorophores, ligands or stimuli-responsive synthetic polymers will be considered at intermediate steps to enable studies and optimization of functional capsules on robust systems. Characterizations will assess stability and shape of membranes (tubes, spheres), density and orientation of scaffold proteins in the membrane, and T-triggered clustering, and permeabilization. Beyond the stage of proof of concept, vast potentialities exist for mass production of bioengineered diagnostic systems (that will be assessed with the company Synthelis), and control of latex/capsules biosynthesis to protect e.g. active compounds in food and cosmetics.

Project coordination

Christophe Tribet (P.A.S.T.E.U.R. UMR8640)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

P.A.S.T.E.U.R. P.A.S.T.E.U.R. UMR8640
CNRS UMR 7099 Laboratoire de biologie physico-chimique des protéines membranaires
CNRS UMR 8531 Photophysique et Photochimie Supramoléculaires et Macromoléculaires
DCM - UMR 5250 Département de Chimie Moléculaire
CNRS UMR 8531 Photophysique et Photochimie Supramoléculaires et Macromoléculaires

Help of the ANR 494,411 euros
Beginning and duration of the scientific project: January 2018 - 48 Months

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