DS01 - Gestion sobre des ressources et adaptation au changement climatique

BIO-fouling COntrolled Membrane – BIOCOM

BIO-fouling COntrolled Membrane

The control of biofouling is required to improve membranes performances and to reduce high operational cost due to cleaning. Biomimetic membranes represent the next generation of filtration membranes used for water treatment. The project BIOCOM aims to provide an innovative biomimetic membrane with anti-biofouling properties for water treatment by incorporating natural bioactive molecules that act as Quorum Sensing Inhibitors (QSI), such as vanillin.

Innovative suface modifications against biofouling

The project BIOCOM aims to elaborate new anti-biofouling membranes by incorporating a natural bioactive molecule, vanillin as a model QS inhibitor, and characterize their properties and performances in the frame of water treatment. <br />For this purpose, we identify three interconnected steps: <br />i) Elaboration of anti-biofouling membranes using bioactive molecules with QS inhibition effect, <br />ii) Characterization of the modified membrane: the adhesion and biofouling layer, and physico-chemical properties of the modified membrane material <br />iii) Evaluate the stability of the activity of the bioactive molecule and study membrane performances in filtration on long time.

Surface modification by UV grafting
Evaluation of the antibiofouling effect, qualifying and quantifying adhesion and EPS production

Vanillin was adsorbed on PES membrane (3M, MicroPES® 1F PH) by soaking in 3 g/L vanillin solution for 3 h. Vanillin methacrylate (VMA) was covalently bonded onto the PES membrane by UV-grafting. Specific bands of vanillin and grafted VMA have been determined by FTIR-ATR (Nexus Thermo Nicolet). Biofilms have been developed on membrane surface placed on agar plate for growth inhibition assays and biofilm development using Pseudomonas aeruginosa (CIP 103467).
Surface characterization using FTIR mapping highlights differences between the modification approaches. An effective adsorption of vanillin and grafting of VMA at the membrane surface is observed on FTIR mapping, showing a good homogeneity of the deposit layer. However, the efficiency in terms of adhesion and biofilm development inhibition is limited due to the instability of the molecule at the membrane surface. At the contrary, the grafting approach leads to the covalent binding of the VMA. Impact of the membrane surface modification is observed on the bacterial growth.

Development of new strategy for surface modifications.
The effect on the biofilm development has to be further investigated and quantified, especially considering the grafting approach

- Bkhait, S., J.-F. Lahitte, C. Causserand and C. Coetsier (2018). Antibiofouling surface modifications using bioactive molecules. Interfaces Against Pollution (IAP), La Grande Motte, France. Oral Com
- Bkhait, S., J.-F. Lahitte, E. Girbal-Neuhauser, C. Causserand and C. Coetsier (2018). Study of interactions between microorganisms and material at the surface of a membrane with antibiofouling properties. Euromembrane, Valencia, Spain. Poster
- Bkhait, S., Neuhauser-Girbal, E., Lahitte, J.-F., Remigy, J.-C., Causserand, C., Coetsier, C., 2018. Antibiofouling surface modifications using bioactive molecules. 4th International Conference on Bioinspired and Biobased Chemistry & Materials (N.I.C.E. 2018), Nice, France. Oral Com
- Bkhait S, Lahitte J-F, Coetsier C, Causserand C, Etude des interactions microorganismes-matériau à la surface d’une membrane aux propriétés anti-biocolmatantes. 23èmes Journées Information Eaux; 2018 9-11 octobre 2018; Poitiers, France. Poster

Thanks to their high efficiency in water pollution remediation and the high quality of the produced water, membrane processes are considered as improved technology for sustainable management of water resources. However, membrane biofouling is one of the most persistent problem and an obstacle to their usage for water reuse due to high operational cost. The control of biofouling is required to improve membranes performances. Biomimetic membranes represent the next generation of filtration membranes used for water treatment. The project BIOCOM aims to provide the principle design and fabrication of an innovative biomimetic membranes with anti-biofouling properties for water treatment.
For this purpose, we consider to elaborate new anti-biofouling membranes by incorporating natural bioactive molecules that act as Quorum Sensing Inhibitors (QSI), such as vanillin. These molecules are considered acting on the attachment of microorganisms to the membranes as well as the cohesion between microorganisms into the fouling layer and so are expected to control the biofouling establishment at the membrane surface. Vanillin, in particular, induces a reduction of exo-polymeric substances responsible in the biofouling development. The modified membrane would have the ability to limit bio-adhesion and biofouling during filtration process that leads to a better stability of water flux. Therefore, by optimization of operating filtration conditions it would reduce the use of cleaning agents and increase the lifetime of the membrane with reducing costs.
In this project, initial membrane materials are PolyEtherSulfone (PES) and Polyvinylidene Difluoride (PvDF) membranes used in ultrafiltration processes for water treatment. We will test different incorporation approaches to modify membrane materials using bioactive molecules. The anti-adhesion and anti-biofouling effect of the elaborate membrane will be explored considering the mode of incorporation and characterization of the biofouling layer will be performed. The modified membranes will be characterized in terms of physico-chemical properties brought by surface modifications and we will also investigate hydraulic and selectivity performances of the modified material. Finally, stability/bioactivity and performances of the modified membrane will be evaluated in filtration on long term in the frame of tertiary wastewater treatment for reuse.
Several technical and scientific challenges are addressed for this purpose: i) Elaboration of anti-biofouling membranes using bioactive molecules, ii) Characterization of the impact of modifications on biofouling development, on physico-chemical properties of membrane interfaces, and on membranes performances, iii) Evaluation of the modified membrane stability and performances in the frame of water treatment.
Final objective is to achieve the sustainable control of membrane biofouling in water treatment. Improving the stability of the water flux and reducing the frequency of cleaning cycle in filtration processes, we hope to promote the usage of membranes for applications in water reuse.

Project coordination

Clémence Coetsier (Laboratoire de génie chimique)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

LGC Laboratoire de génie chimique

Help of the ANR 248,543 euros
Beginning and duration of the scientific project: September 2017 - 36 Months

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