Chemistry of Renewable Carbon for Bio-Fertilization & Plant Nutrition – C2R-BioNut

METHODS:
I - in synthetic chemistry methods: 1) urethanisation one-pot or in two stages of glycerol to glycerol carbonate; 2) acylation of glycerol carbonate systems organized by esterification and transesterification in the synthesis of glycerol carbonate esters; 3) oligomerization chemical couplers glycériliques cyclocarbonate (glycerol carbonate esters) by opening the 5-membered ring in autogenous reactor and triphasic liquid / solid system / gas
II - Characterization Methods: cross analysis and characterization of the structures of glycerol carbonate esters and oligocarbonate and oligoglycerol acylated by 1H NMR, 13C, FTIR, MS, MALDI-TOF
III - Methods by complexing interaction between cyclic carbonates and metals in vitro and in silico
IV - in silico modeling of the potential use of cyclic carbonates in the vectorization of metals
V - Formulation and controlled release of agro-materials through the development of concentrated suspensions
APPROACHES:
I - Concept dual «Green Chemistry - Agronomy«
II - The triptych «Green Chemistry - Biotechnology« Agronomy «

In chemical synthesis, design and realization of the building blocks according to the concept of chemistry C3 glycerin / glycerol carbonate has achieved the chemical structural diversity of monomers and oligomers that are compatible with the chemical structural diversity of leaf surfaces. It is thus introduced functionality and technofonctionnalité in the glycerol based C3 structures and glycerol carbonate or oligomers obtained by outgrowth of the glycerol backbone homo-oligocarbonate glycerol, oligoglycerol homo-and co-oligocarbonate / oligoglycerol. In vitro and in vivo the concept of adjuvantation - complexation - vectorization is demonstrated through interactions between glycerol carbonate esters and biomaterials and in the formulation of suspension concentrates Mn. The transport mechanism of the active materials - biomaterials and / or microorganisms results in bio-control growth and plant development through the use of native formulations based emulsions and / or chemical couplers glycériliques which revealed eliciting properties in plants.

In synthetic chemistry, bio-inspired by the chemical structural diversity of leaf surfaces guides our work towards the design and development of chemical couplers glycériliques cyclocarbonates, oligomers and dendrimers carboxylic termination. These new candidates will be studied via complexation understanding interactions with metals by modeling methods in vitro and controlled release in vivo methods of biomaterials. Topics will compare the robustness of candidates for termination hydroxymethyl compared to candidates carboxylic termination in their role as transportation and control of the development of plant growth subject to biotic and abiotic stress.

MOULOUNGUI Z. ATTIA F. DUMAS B. Procédé de conservation de micro-organismes à température ambiante et composition. FR 14.62208 10/12/2014
MOULOUNGUI Z. DUMAS B. ATTIA F. Composition antiseptique par contact et procédé non thérapeutique de traitement antiseptique par contact. FR 14.62205 10/12/2014
MOULOUNGUI Z. LACROUX E. DUMAS B. BELMAS E. ATTIA F. Procédé de préparation d’une composition de traitement de plante, composition obtenue et ses utilisations. FR 14.62200 6 10/12/2014
ABDEL Z. MOULOUNGUI Z. CABANES C. ATTIA F. GUEUNIER M VALENTIN R. ZEBIB B. Utilisation d’esters carboniques de glycérol en agriculture. FR 3 003 130 (19/09/2014)
VALENTIN R. HOLMIÈRE S. MOULOUNGUI Z. Esters of Oligo-(Glycerol Carbonate-Glycerol Ether) as Alternative to Fossil Surfactants. 7th Workshop on Fats and Oils as Renewable Feedstock for the Chemical Industry. Karlsruhe (Allemagne) March 23 – 25 2014
VALENTIN R. NYAME MENDENDY G. MOULOUNGUI Z. Physico-chemical understanding of the synthesis of partial glycerol and diglycerol esters of undecylenic acid. 106th AOCS Annual Meeting and Industry Showcases. Orlando, Floride (USA), 3-6 may 2015
THIEBAUD S. BERGEZ M. DE CARO P. FABRE J.F. GERBAUD V. MOULOUNGUI Z. Substitution methodologies to design new bio-based solvents. International Workshop on Alternative Solvents for Extraction, Purification and Formulation. Avignon, 4 Juin 2015
ATTIA F. ABDEL Z. VALENTIN R. ZEBIB B. MOULOUNGUI Z. Do bionik molecules of plant cuticle act as biostimulant through their multifunctionnalties. 2nd World Biostimulants Congress. Florence (Italie), 16-19 nov 2015
BERGEZ M. THIEBAUD S. DE CARO P. FABRE J.F. GERBAUD V. MOULOUNGUI Z. From chemical platform molecules to new biosolvents: Design engineering as substitution methodology. BIOFPR, 2014, 8(3), 438-451
FABRE J.F. LACROUX E. VALENTIN R. MOULOUNGUI Z. Ultrasonication as a highly efficient methode of flaxseed mucilage extraction. Ind. Crops Prod. 2014, doi.org/10.1016/j.indcrop.2014.11.015

C2R-BioNut is a joint Laboratory between Laboratoire de Chimie Agro-Industrial - CAI UMR 1010 INRA / INP-ENSIACET and Agronutrition Company. The main objective is to develop innovative solutions of biofertilization for Plant Nutrition towards sustainable agriculture. The stating date is on June 2014. The challenge is to maintain a high level of agricultural production while reducing the environmental impact due to changing climate in order to ensure food and nutrient security by sustainable ways. Three strategies are implemented: two dual-type strategies: Green Chemistry/Agronomy, Biotechnology/Agronomy and one triptych strategy based on green chemistry/biotechnology/agronomy. These multidisciplinary approaches aim to global synergistic effect on plant nutrition.
? Strategy I: Green Chemistry/Agronomy. Renewable carbon chemistry uses glycerol as a green carbon source. Glycerol is well known as the main bi-product of oleo chemical and biofuel industries. In our research program glycerol is the starting material used to investigate original green synthetic pathways to obtain multifunctional glycerol cyclocarbonates, monomers and oligomers (linear and / or hyperbranched) of glycerol cyclocarbonates moreover to obtain macromolecules dendrimers - like. These new biobased materials offer opportunities to develop magneto- rheological electro-rheological fluids; these characteristics are enhanced while interacting with macro-, meso- and micro-nutrients. We propose developing new technologies in particles organizational systems using specific structures molecules, hybrid, organic-inorganic, liquid to solid reversible state, evolving structures with adjuvantation properties compatible with agricultural inputs. These innovative synthons offer the opportunity to study the "Host / Guest" concept. By specific research on this concept we will carry out further investigations on complexation, adjuvantation, vectorization and controlled and slow-release of ingredients.
? Strategy II. Biotechnology/Agronomy and the control of sustainable agronomic inputs by the associative approach between cultivated Plant and beneficial microorganisms. The Biotechnology / Agronomy program of Agronutrition Company has specifically developed biofertilizers, which are based on three types of microorganisms, actinomycetes, azotobacters and mycorrhizae as innovative solutions. These biofertilizers promote plant growth for example by increasing the uptake of nutrients and production of phytohormones. Several key scientific and technological questions remain unresolved in this topic and require additional efforts for the adjuvantation, formulation, vectorization of bioactive compounds and microorganismes. The eeconomic development of these products is mainly hampered by the lack of this type of solutions. They could be largely resolved by technological advances in the chemistry of glycerol and renewable carbon.
The research activities carried out within the Agronutrition’s biotechnology Lab contribute to the acquisition of new knowledge on the impact of these microorganisms on the plant physiology and crops yields and quality. These findings are subject to industrial valorization to optimize crops as sources of green and sustainable carbon.
Strategy III. Green Chemistry/Biotechnology/Agronomy. Based on glycerol chemistry as a renewable source of carbon we suggest a second exploratory model to exalt synergies and specific molecular signatures to enhance plants behaviors to mitigate biotic and abiotic stress and external stimuli. In addition, membrane components (oleosomes/oleosins, phospholipids, sphingolipids, sterols ...) will be used due to their intrinsic techno - functional properties and their richness of native trace elements. These smart mimetic bioprocesses are another innovative and alternative ways to preserve healthier plants, with higher nutritional status that satisfies sustainability requirements.