Référence projet : 11-EQPX-0008
RST : Michael NILGES
Etablissement Coordinateur : Institut Pasteur
Région du projet : Île-de-France
Discipline : 5 - Bio Med
Aide de l'ANR 7 515 770 euros
Investissement couvrant la période de septembre 2012 à décembre 2019
Cells function through transient interactions of macromolecular complexes. Understanding fully the dynamic processes of life at the molecular level requires understanding the structures of these dynamic complexes. Developing the tools to accomplish this aim is the subject of CACSICE: we are creating a centre dedicated to studying the spatio-temporal complexity of macromolecular assemblies and macromolecules in living systems. Historically, structural biology has been largely confined to studying static structures of a single molecule with a single technique, in most cases X-ray crystallography. To understand the formation and evolution of transient complexes within a living cell, we need a radical change in structural biology approaches. Data acquired with multiple biophysical techniques at multiple scales must be collected and integrated into one consistent, dynamic, picture that relies both on emerging experimental technologies and on modelling and numerical simulations. To put this multi-site platform into operation is in itself a challenging task. The first technologies are in operation, notably a novel mass spectrometer that allows one analyse the large dynamic molecular complexes; also, through the installation of a new direct detection camera, the obtainable resolution of a 200 kV microscope has been dramatically increased. Further major installations are foreseen: a 700 MHz solid / liquid state NMR spectrometer has recently been installed at the IBPC in 2016; and the Titan Krios 300 kV microscope will be installed starting in April 2017 at the Institut Pasteur in a new dedicated building.
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