Theranostic treatment of ischemic stroke using magnetic hyperthermia – ISCHeMAG

The challenge of this project is the development of a multimodal treatment against thrombosis (stroke) using innovative theranostic inorganic nano-objects. Our approach is based on the design of biodegradable nanomaterials of core@shell type (iron oxide@porous silica with large pores) allowing a localized hyperthermia action, combined in a first strategy with the delivery of antithrombotic protein (tPA) and in a second strategy with a neuroprotective antioxidant effect.
This project is structured on the judicious complementarity of a consortium bringing together : i) the nanoengineering of degradable mesoporous silica around activatable iron oxide cores (IPCMS, CNRS, Strasbourg) allowing magnetic hyperthermia and optimized protein loading/delivery from these core@shell nano-objects (size 50-100 nm); ii) the synthesis of small inorganic nanoparticles (size 2-10 nm) grafted or incorporated into the pores of the core@shell NPs, namely on the one hand quantum dots (QDs) making it possible to evaluate the local temperature of the core@shell (nanothermometry) and used as measurement tools and on the other hand platinum nanoparticles having an antioxidant effect ensuring the neutralization of radical species (ROS) deleterious to antithrombotic treatment (LPCNO, INSA, Toulouse) and iii) the biological evaluation of these systems on in vitro models of thrombosis obtained from human blood and ultimately of their thrombolytic action improved by magnetic hyperthermia on a mouse model of ischemic stroke (PhIND, INSERM, Caen).