Chromosome Morphology and Homologous Recombination in meiosis: a new role for condensin? – CoMoRe

In sexually reproducing organisms, the transmission of a complete and intact genetic material from one generation to another depends on the specialized cell division of meiosis, which leads the formation of the haploid gametes. During this division, the DNA repair process of Homologous Recombination is essential for the correct segregation of the homologous chromosomes, as it allows the formation of a physical link between the homologs. Therefore, at the onset of meiosis, numerous DNA double strand breaks are formed and repaired by Homologous Recombination. Importantly, meiotic Recombination must be tightly regulated to avoid the appearance of mutations or chromosomal rearrangements during breaks repair, that can potentially be transmitted to the offspring.
Meiotic Recombination occurs in the context of a complete structural reorganization of the chromosomes, which is essential for a proper segregation of the homologs. Nevertheless, the factors that connect this reorganization of the chromosomes to Homologous Recombination remain poorly understood.
Our preliminary data suggest that SMC-condensin complexes, known for their role in the organization of the chromosome, could act as a regulator of meiotic Recombination. The following proposal aims at studying the role of condensins in the control of chromosome structure and Recombination in meiosis. We propose to developpe a multi-pronged approach designed to characterize the phenotype of condensin mutant mice, in order to determine their role during meiosis.
Therefore, this project offers a unique opportunity to characterize the function of a new factor, condensins, in the control of chromosome structure, Homologous Recombination and genetic stability, during gametes formation in mammals.