Extracellular matrix-mediated force propagation at a tissue/tissue interface – MECAMATRIX

The biomechanics of morphogenesis must be understood not only in the scope of isolated tissues, but also in the framework of interacting tissues. Our aim is to decipher how the extracellular matrix (ECM) transmits mechanical forces between neighbouring tissues during morphogenetic movements. To tackle this question, we will study the ECM-mediated mechanical interplay between the olfactory placode and eye tissues in zebrafish, which develop in a superficial location amenable to live imaging and mechanical perturbations. With an interdisciplinary strategy combining biology with physics, we will (1) analyse ECM architecture and dynamics at the tissue interface using quantitative imaging, (2) probe forces and local material properties in the ECM using engineered oil droplet sensors and laser ablation, and (3) finely tune ECM mechanics/composition at the tissue interface with genetic/pharmacological approaches, to analyse the effects on the tissue/tissue coupling. This work will bring new mechanistic knowledge on the role of ECM and its mechanical properties in tissue morphogenesis in vivo, with potential implications for ECM-related diseases, tissue engineering and regenerative approaches.