CE44 - Biochimie et chimie du vivant

Single Granule oMics to support starch synthetic biochemistry – SinGraMics

Submission summary

Starch is a main storage form of carbon for plants. It is composed by glucose polymers forming water insoluble granules that harbour a wide range of sizes and morphologies regarding to their origin (e.g., plant species, organs, tissues, cells). In addition, starch granules contain minor fractions of proteins, lipids and phospholipids as well as phosphate groups covalently bound to glucose moieties. Starch is an important raw material for food and non-food industries and represents a promising way for atmospheric carbon capture. In 2021, starch-like polymers were successfully synthesized chemoenzymatically from atmospheric carbon dioxide and hydrogen in vitro. However, no evidence for a granular, semi-crystalline packed organization neither for mimicking starch physicochemical properties were presented. This raises the questions about which additional natural components (e.g., structural proteins, phosphoesters) are necessary for native starch synthesis and how to normalize synthesis steps to produce homogenous starch granules. In previous projects, we developed methods for studying miniaturized starch samples and, to date, could characterize glucan length distribution and the proteome of single potato starch granules. We also established methods for the structural analysis of phosphoglucans and miniaturized lipidomics. With this project these developments will be finalized and used to characterize single starch granules and investigate their heterogeneity as well as the contribution of minor compounds to granule architecture. Establishing the relationships between granule composition, structure and morphology at the level of single granules will help feeding mathematical models contribute in designing homogenous artificial synthesis workflows.

Project coordination

Nicolas Szydlowski (Unité de Glycobiologie Structurale et Fonctionnelle)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.


UGSF Unité de Glycobiologie Structurale et Fonctionnelle
MSAP Miniaturisation pour la Synthèse, l'Analyse et la Protéomique

Help of the ANR 433,617 euros
Beginning and duration of the scientific project: December 2022 - 42 Months

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