CE13 - Biologie cellulaire, biologie du développement et de l’évolution

Mechanisms of deterministic and self-organised dendritic neuron morphogenesis – DendroMorph

Submission summary

Formation of dendritic arbors is crucial for neural circuit functioning. Despite more than 100 years of characterization, the mechanisms governing dendrite morphogenesis are still not fully understood. Dendrite development is commonly described as a deterministic process, governed by genetically encoded cues that specify branch position and growth direction. However, recent progress in live imaging techniques have revealed that the core morphology of dendritic arbors also largely relies on highly dynamic stochastic processes, which allow the exploration of many morphological configurations. Using Drosophila dendritic arborization (da) neurons formation as a model system, we aim to understand how deterministic and stochastic self-organized mechanisms interplay to define the unique shape of each neuron. We will pursue the work we have initiated on simple c-I da neurons and extend it to complex c-IV da neurons. Using a combination of high-resolution live-imaging, computational modelling, gene candidate/RNAi approach and single cell RNA sequencing we will: (i) determine how c-I and c-IV dendritic shape emerges from local branching dynamics, (ii) identify the molecular and mechanical effectors controlling these dynamics and (iii) identify the intrinsic genetic programs specifying the morphology of each class.

Project coordination

Thomas Lecuit (Centre National de la Recherche Scientifique_Délégation Provence et Corse_Institut de Biologie du Développement de Marseille)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

CNRS DR12_CPT Centre Nationale de la Recherche Scientifique_Délégation Provence et Corse_Centre de Physique Théorique
CNRS DR12_IBDM Centre National de la Recherche Scientifique_Délégation Provence et Corse_Institut de Biologie du Développement de Marseille

Help of the ANR 562,943 euros
Beginning and duration of the scientific project: December 2022 - 48 Months

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