Structure diversity, functionality and modulation of milk oligosaccharides in monogastric livestock species: towards optimal development of rabbit and pig holobionts – HoloOLIGO
As the first available prebiotics for neonates, milk oligosaccharides regulate gut microbial composition and modulate host immune response, playing a crucial role in the holobiont assembly. By using two livestock models (pigs and rabbits) with different maturity levels at birth, HoloOLIGO aims to decipher causal links between milk oligosaccharidesstructures, the offspring microbiota and immune system. We will create a database using data mining of the literature to find, visualise and analyse milk oligosaccharidesstructure diversity patterns within and between mammalian species. We will produce the first MO data in rabbits and expand them in pigs. To understand structure importance of milk oligosaccharides, we will undertake in vitro functional analyses in both species on commensal bacterial strains and intestinal immune cells and further validate results in vivo. Finally, we will evaluate, via in silico analyses (pig) and in vivo (rabbit), the existing genetic variability and assess the genetic determinism of milk oligosaccharidescomposition.
Project coordination
Sylvie Combes (Génétique Physiologie et Systèmes d'Elevage)
The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.
Partner
SPI/LEMM Service de Pharmacologie et d'Immunoanalyse
MaIAGE Mathématiques et Informatique Appliquées du Génome à l'Environnement
GABI Génétique Animale et Biologie Intégrative
PEGASE Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage
MICALIS MICrobiologie de l'ALImentation au service de la Santé
GenPhySE Génétique Physiologie et Systèmes d'Elevage
3P Physiologie et Phénotypage des Porcs
Help of the ANR 687,257 euros
Beginning and duration of the scientific project:
March 2022
- 48 Months
