CE12 - Génétique, génomique et ARN

Regulation of Mammalian Enhancers by DNA methylation – REMEDY

Submission summary

Cellular function depends on a precise pattern of gene expression, and alterations of this process are linked to human diseases. This pattern is determined by interactions between transcriptional regulators, regulatory elements (promoters and enhancers), and the cell’s epigenetic landscape. Three key epigenetic marks are chromatin accessibility, histone modifications, and DNA methylation. In spite of existing data, an important question that is not fully understood is the role of DNA methylation in mammalian enhancer activity. Does DNA methylation contribute directly to enhancer repression? Does it activate a subset of enhancers? What are the mechanisms underlying repression and activation? To answer these questions, we will use advanced molecular biology and genomics, in a physiological cellular system where methylation is dynamic and genetic tools available. We will also develop a new technique at single-molecule resolution to go beyond correlations and population-based assays.

Project coordination

Pierre-Antoine DEFOSSEZ (Epigénétique et destin cellulaire)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

TAGC Théories et approches de la complexité génomique
Epigénétique et destin cellulaire
IJM Institut Jacques Monod

Help of the ANR 582,108 euros
Beginning and duration of the scientific project: September 2021 - 42 Months

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