Blanc SVSE 5 - Blanc - SVSE 5 - Physique, chimie du vivant et innovations biotechnologiques

Auxin-dependent dynamics of cell wall xyloglucans – AuxiWall

Auxin-dependent dynamics of cell wall xyloglucans

In this project we want to identifiy the actors of biosynthesis and remodeling of Xyloglucan that are controled by the Auxin and how does this fine tuning of cell wall structure does impact physical properties of the cell wall and consequently the cell elongation.

How hormone signal is “translated” into cell wall modification inducing anisotropic expansion ?

Our project focuses on the cell wall, and more specifically on the hormonal control of the xyloglucan quality. The acquired data will lead to a better undestanding of the regulation of cell expansion in plants.<br />We anticipate that the knowledge provided within this project will also provide valuable data for the green chemistry derived from ligno-cellulosic biomass being directly dependent on both cell wall quality and plant growth.

Cytological and biochemical methods will be scaled down to allowed tissue specific analysis. These various approaches will be combined to dynamically vizualized in vivo the plant cell wall modifications occuring during cell elongation.

We have shown the mandatory role of post-synthesis cell wall remodeling. We revealed that this remodeling is needed for a proper elongation and that auxin does play a role in controlling this remodeling. We also showed an unexpected cellular trafficking of polysaccharides in elongating cell. Finaly we developped new tools for the in vivo labelling of cell wall polysaccharide. A highly innovant tool for plant biologists.

Our project will bring knowledge about the mechanism of plant growth and will lead to a better understanding of cell wall biosynthesis. This knowledge will provide valuable data needed to improve process of ligno-cellulosic biomass to non food products.

1) Sechet J, Frey A, Effroy-Cuzzi D, Berger A, Perreau F, Cueff G, Charif D, Rajjou L, Mouille G, North HM, Marion-Poll A. (2016). Xyloglucan Metabolism Differentially Impacts the Cell Wall Characteristics of the Endosperm and Embryo during Arabidopsis Seed Germination.Plant Physiol. 170(3):1367-80. doi: 10.1104/pp.15.01312.
2) Dumont, M., Lehner, A., Vauzeilles, B., Malassis, J., Marchant, A., Smyth, K., Linclau, B., Baron, A., Pons, J.M., Anderson, C.T., et al. (2016). Plant cell wall imaging by metabolic click-mediated labelling of rhamnogalacturonan II using azido 3-deoxy-D-manno-oct-2-ulosonic acid. Plant Journal 85, 437-447.
3) Dumont, M., Lehner, A., Bardor, M., Burel, C., Vauzeilles, B., Lerouxel, O., Anderson, C.T., Mollet, J.-C., and Lerouge, P. (2015). Inhibition of fucosylation of cell wall components by 2-fluoro 2-deoxy-L-fucose induces defects in root cell elongation. Plant Journal 84, 1137-1151.
4) Paque, S., Mouille, G., Grandont, L., Alabadi, D., Gaertner, C., Goyallon, A., Muller, P., Primard-Brisset, C., Sormani, R., Blazquez, M.A., et al. (2014). AUXIN BINDING PROTEIN1 Links Cell Wall Remodeling, Auxin Signaling, and Cell Expansion in Arabidopsis. Plant Cell 26, 280-295.
5) Chen, X., Grandont, L., Li, H.J., Hauschild, R., Paque, S., Abuzeineh, A., Ralcusova, H., Benkova, E., Perrot-Rechenmann, C., and Friml, J. (2014). Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. Nature 516, 90-U206.

name : on contract on this project

Many communications in congress (poster and conference).

Plant cells are surrounded by a complex and dynamic carbohydrates-formed cell wall that is essential during development for cell expansion and shape, and contributes to plant architecture, cell protection and production of biomass. The primary cell wall is formed by a network of crystalline cellulose microfibrils interlinked with hemicelluloses and embedded in a matrix of pectins including various proteins. During growth, constant reorganizations of the primary cell wall have to occur to allow both changes in extension capabilities and integration of novel material. Growth is regulated by plant hormones, amongst which auxin is a key regulator of cell expansion. However, how auxin influences cell wall modification during growth remains a central but unresolved question. Primary data, generated by the partners, suggested that the auxin receptor ABP1 is involved in the control of hemicellulose modifications and more specifically in the branching of xyloglucans; thus pointing out ABP1 as an essential component promoting auxin-dependent modifications of the cell wall. In the project, we propose to investigate the dynamic changes of xyloglucans during cell elongation and to determine the influence of auxin on this process. Analyses of dynamic changes in xyloglucan will be achieved using recently developed high spatial resolution and high throughput mass spectrometry (P1). Further understanding of xyloglucan modifications will be addressed by developing labeled molecules using click chemistry for in vivo labeling and visualization. Plants exhibiting the conditional loss of function for ABP1 with altered xyloglucans will be used in combination with available Arabidopsis genetic resources (mutants affected in auxin signaling and mutants affected in xyloglucan biosynthesis) to investigate how auxin affects xyloglucans and how xyloglucan modifications affect cell elongation.

Project coordination

Grégory MOUILLE (INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE - CENTRE DE RECHERCHE DE VERSAILLES GRIGNON) – gregory.mouille@inrae.fr

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

INRA-AgroParisTech INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE - CENTRE DE RECHERCHE DE VERSAILLES GRIGNON
ISV - CNRS CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - DELEGATION REGIONALE ILE-DE-FRANCE SECTEUR SUD
LG2M-ICMMO UNIVERSITE DE PARIS XI [PARIS- SUD]

Help of the ANR 428,582 euros
Beginning and duration of the scientific project: - 36 Months

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