JCJC SVSE 6 - JCJC : Sciences de la vie, de la santé et des écosystèmes : Génomique, génomique fonctionnelle, bioinformatique, biologie systémique

Perception of lipo-chitooligosaccharides and roles of their putative receptors in non legumes – LCOinNONLEGUMES

Perception of beneficial microorganisms by plants

Plants interact with some beneficial soil microorganisms (bacteria and fungi) which provide them with nutrients. These interactions require recognition by plants of chemical signals produced by the microorganism.

Identification of plant genes involved in the perception of microorganisms.

Lipo-chitooligosaccharides (LCOs) are chemical molecules secreted by symbiotic bacteria called rhizobia. These LCOs are essential for the establishment of the nitrogen-fixing symbiosis between rhizobia and roots of plants belonging to the legume family. Recently it has been shown that LCOs are also produced by symbiotic fungi called arbuscular mycorrhizal fungi, which colonize the roots of most plants and which improve plant nutrition. LCOs have positive effects on plant development and growth and are therefore of agronomic interest. Studies on LCO perception mechanisms in plants will allow optimization of plant responses to LCOs and interactions between plants and these symbiotic microorganisms.

Proteins that bind to LCOs have been detected using traceable, modified LCOs. These proteins will be purified and identified by spectrometric techniques. The genes encoding these proteins will then be isolated. The LCO binding properties and the roles of these proteins in the interactions between plants and microorganisms producing LCOs will be analyzed in detail.

under progress

under progress

under progress

Lipo-chitooligosaccharides (LCOs) secreted by rhizobia are essential for establishment of the nitrogen fixing symbiosis with their host legume. In addition to the well known effects on legumes, LCOs have also been reported to elicit responses in non-legumes and LCOs are now sold for corn treatment, as plant growth stimulating factors. I have detected LCO binding proteins in non legume cell cultures and have observed non symbiotic responses following expression of putative LCO receptors in non legumes. The objective of the project is to identify and determine the role of LCO binding proteins in non legumes and to understand the non symbiotic function of putative LCO receptors. I plan to purify proteins cross-linked to LCOs and analyse them by MS/MS and then to validate their function in LCO binding and in eliciting LCO effects in non legumes. Concerning the non symbiotic functions of putative LCO receptors, I plan to identify the nature of stimuli inducing the responses and the signalling pathway leading to these responses.

Project coordination


The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.



Help of the ANR 205,102 euros
Beginning and duration of the scientific project: - 48 Months

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