JCJC - Jeunes chercheuses & jeunes chercheurs

Le mouvement sans actine et myosine : motilité amoeboide des spermatozoïdes de Caenorhabditis elegans – Elegant sperm

Submission summary

Moving without actin and myosin: sperm cell crawling in Caenorhabditis elegans Mammalian cell movement is powered by actin polymerization, in concert with molecular motors like myosin that confer contractility to the cell. The sperm cells of the nematode Caenorhabditis elegans contain a 'Major Sperm Protein' (MSP) cytoskeleton, which plays the role of actin in these cells, despite the fact that MSP has no sequence, structural or biochemical homology to actin. The equivalent of myosin in nematode sperm cells is unknown. The goal of this project is to identify the proteins that orchestrate MSP dynamics by a dual biochemistry (immunoprecipitation, GST pulldown)/genetics (RNAi) approach. With this information, we plan to develop a minimal set-up to reproduce sperm cell movement in vitro. This system will allow us to probe the biochemical and physical parameters of MSP assembly and MSP-based propulsion. In parallel, we will study sperm cell movement in vivo by producing a transgenic GFP-MSP worm that will allow us to visualize the MSP cytoskeleton in a moving cell. We will study the mechanisms of adhesion, lamellipodial extension and symmetry breaking, and compare our results to theoretical models concerning these subjects. By studying MSP dynamics, we hope to uncover novel mechanisms for cell polarization and cytoskeleton organization, and to reveal the governing principles of cell motility.

Project coordination

Julie Plastino (INSTITUT CURIE - SECTION DE RECHERCHE)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

INSTITUT CURIE - SECTION DE RECHERCHE

Help of the ANR 151,320 euros
Beginning and duration of the scientific project: - 36 Months

Useful links

Explorez notre base de projets financés

 

 

ANR makes available its datasets on funded projects, click here to find more.

Sign up for the latest news:
Subscribe to our newsletter