MUSCOVADO: MUlticale SCaffOld-free and VAsculariseD Organoid-based tissue: fabrication & comprehension – MUSCOVADO

Regenerative medicine is a burning stake for public health. The growing expectations for tissues that are engineered from patient’s cells and no longer from donors is a major challenge. Although culturing small pieces of tissues in vitro is nowadays mastered, scaling up the process in terms of tissue size hasn’t been achieved yet, mainly because this implies providing nutrients to each cell of the construct. This observation points vascularisation as the main key to unlock the situation.
The MUSCOVADO project ambitions to provide a framework to engineer biological tissues that could be grown up to centimetre-size, by means of its design that integrates a multiscale vasculature. Briefly, the approach consists in assembling building-blocks of micro-vascularised tissues with meso-scaled engineered vessels that we hypothesise to connect to each other. This is directly applicable to medicine, but also serves to investigate angiogenesis and vasculogenesis in a relevant in vitro system.