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Rac1 signal multiplexing : a quantitative approach from in-vitro to in-vivo – MULTIPLEX
this issue by quantitative approaches merging in vitro and in cellulo systems, focusing on the activation [...] and quantify these parameters by integrating in vitro kinetics assays using purified proteins and controlled
Engineered 3D brown-like adipocytes derived from human iPSCs for in vitro preclinical drug discovery and for cell-based therapy to treat obesity – hiPSC-Adipospheres
To counteract obesity and associated metabolic disorders is a public health challenge. The very low success rate in clinical trials, and the discovery of new drugs being a slow and expensive process, it is necessary to propose an innovative and alternative approach to fight this disease. We propose novel in vitro preclinical models of human beige adipose tissue organoids for drug screening and biotherapy.
The IntraFlagellar Transport machinery: regulation of motors and microtubules dynamics in vitro. – IRMM
Intraflagellar transport proteins (IFTs) are essential for microtubules (MTs) organization both inside and outside of the cilium, through their interactions with MTs and/or MT associated motors. However, due to the complexity of the IFT machinery a lot of work remains to be done to precisely characterize those molecular mechanisms. Here, to circumvent this complexity, we propose to use simplified in vitro systems to reduce the molecular complexity of the cellular context.
Water-soluble aza-BODIPY platform for multimodal imaging and trackable therapeutics – WazaBY
Development of a new class of water-soluble fluorophores, optimized for near infrared imaging (NIR).<br />Functionalitzation of the boron center on the aza-BODIPYs, in order to develop new bimodal and theranostic agents.<br />Valorization of the systems by a preclinical study, aiming at the bimodal imaging and theranostic approach of cancers overexpressing PD-L1.
Upconversion nanoparticles for in vivo NIR light-induced Drug Release – LightinDR
efficient red to blue upconversion. The in vitro and in vivo efficacy of upconversion assisted photolysis
Role of Type IV pilus retraction in bacterial pathogenesis – RETRACTOPATH
Type IV pili (Tfp) are highly dynamic long filamentous structures that are produced by numerous pathogenic bacteria. They mediate an extraordinary array of functions, including motility, DNA uptake, bacterial aggregation or adherence to host cells. Pilus retraction generates remarkably high force. Such force acts as a mechanical signal to both bacteria and host cells. To date how such force is sensed in host cells and contribute to bacterial pathogenesis has been poorly explored.
A novel multi-stage acting antimalarial drug-candidate – plasmodrug
. This original molecule displays excellent in vitro activities even on the artemisinin-resistant parasites [...] solubility, which limit gamhepathiopine activity in vivo. In this context, we thus propose to pharma [...] pharmacokinetic properties and to potentiate its in vivo activity. To this aim, the metabolic stability issue
Morphogenesis of two active tissues in contact, a multi-scale mechanical analysis – EpiMorph
focused on how specific cell types behave in vivo or in vitro, but had rarely integrated the input of different [...] predicted from in vitro studies regulates the mechanical response of tissues in vivo, and by proposing [...] but such studies have seldom been validated in vivo. Finally, while several theoretical studies have
Meningococcal Infections-on-Chip: Integration of the Tissue Architecture – MeningoChip
ied in vitro models (2D monolayers of endothelial cells or Transwell) or on a complex in vivo human skin [...] structure and the function of Nm-target tissues in vitro, in order to investigate the course of meningococcal [...] strategies has permitted the design of advanced in vitro assays such as perfusable 3D microvascular networks
Presubicular bursting and visual anchoring of the head direction signal – BURST
How the brain encodes spatial orientation depends strongly on vestibular and visual signals, but the cellular and circuit basis for visual anchoring of the internal compass remains unknown.<br />We will focus on the presubiculum, and the feedback to the lateral mammillary nucleus, that seems to rely on intrinsic bursting neurons of layer 4. Our project evolves around the idea that a burst feedback signal has a key role for visual updating of the head direction signal.