High-resolution microscopy techniques are one of the fundamental tools in biomedical imaging studies.
"Nobelized" work of Bertzig and Hell in PALM/STED/STORM have paved the way to high-resolution microscopy (down to 50 nm).
However, these methods need the addition of a fluorescent dye to operate, which can be a limiting factor when in-vivo imaging is considered.
Recently, the development of interferometric methods like tomographic diffractive microscopy has shown that it was possible to exact a 3D structural information without using contrast agent. Moreover, using heterodyne interferometry makes it possible to obtain a dynamic information about the sample (2D Doppler effect) and thus discriminating static from dynamic structures.
We here propose to couple both techniques in a new imaging device aiming at imaging, without contrast agent, cellular dynamics (3D Doppler effect). In order to cope with the acquisition time issue, stress will be put to both experimental improvement (use of high-speed cameras and illumination scanning device), and numerical aspects (improvement of the reconstruction algorithms so as to limit the amount of acquired images for full resolution characterization).
At the end of THTTM project, it will be possible to perform 3D + Doppler contrast imaging, without contrast agent, and on moving object with a frame rate around 10 Hz. We hope these developments will be insightful for living sample characterization.
Monsieur Nicolas Verrier (Institut de Recherche en Informatique Mathématiques Automatique Signal (IRIMAS) - EA 7499)
The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.
IRIMAS Institut de Recherche en Informatique Mathématiques Automatique Signal (IRIMAS) - EA 7499
LP2N Laboratoire Photonique, Numérique, Nanosciences
Help of the ANR 260,712 euros
Beginning and duration of the scientific project: December 2019 - 48 Months