Opuntia ssp as a source of novel compounds for pharmaceutical new products development. Characterization of bioactive compounds (phytochemicals and biopeptides) and their changes through plant domestication.
Metabolite and protein characterization of Opuntia wild and domesticated species. Peptide charecterization and their possible bioactivity. Cell culture of wild Opuntia species for the production in mass of metabolites and/or peptides.Bioactivity on different physiological and pathological end-points of Opuntia specied with various domestication degrees.
For the various Opuntia species with different domestication degree:
Classical proteomic analyses for proteins; shot gun for peptades after enzymatic hydrolysis.
Assay of bioactive phytocompounds
Bioactivity on in vitro cell cultures and on in vivo animal models
Opuntia cell culture
Some peptides are different as a function of the domestication degree.
A light protective role on end-points linked to atherosclerosis, with in vitro tests
No effect on colorectal carcinogenesis with in vitro tests
In vitro culture of Opuntia is possible.
In vivo tests
“Antioxidant properties of wild and domesticated Opuntia Species. Potential interest in atherosclerosis”
Corinne Bernis, Ana Paulina Barba de la Rosa , Michel Rossignol, Françoise Guéraud, Robert Salvayre, Anne Nègre-Salvayre
Society for Free Radical Research International, London September 6-9 2012. Poster
Medicinal herbs have been used for many thousands of years as alternative therapies to complementary medicines. Hence, there is an increasing demand for Traditional Herbal Medicine (THM). Pharmaceutical companies are keen to identify single bioactive compounds that are extracted from THMs. However, the multi-component and synergistic nature of THMs means that it is beneficial to analyze complex extracts.
Among the several Mexican plants that have been claimed as medicinal, Opuntia spp. is one with higher biotechnological potential : they can grow in arid areas, are feed and food resources, and the most important, it has been used as a folk medicine for the treatments of diabetes, gastric ulcer, inflammatory, and several other illnesses. But still the compound(s) that are responsible of the beneficial effect are not well known. In addition, there are not reports about the possible biopeptides participation on the medicinal effects of Opuntia plants. The lacks of information about type of proteins presents in cladodes are mainly due to technical manipulation of protein extraction. Nowadays, modern technologies, as higher resolution HPLC columns, powerful software’s, and the ability to determine the amino-acid sequences by Mass Spectrometry, gives the tools to analyse hundreds or even thousands of individual chemical entities present in a wide range of concentration levels with the result of a better characterization of proteins (peptides) and phytochemical compounds. One of the challenges for use plants as source of active compounds is, that the active compounds are not enough for a treatment, or the technical processing during products elaboration has a detrimental effect on the active molecules. For this reason, plant cell culture has great potential as an alternative system for the production of phytochemicals normally extracted from whole plants. This system can overcome many of the problems associated with industrial production of these phytochemicals extracted from field grown plants. In cultures, the production of natural compounds can be carried out throughout the year, unaffected by the season. The risk of crop failure due to natural hazards and the danger of extinction of some species due to their mass extraction from natural populations are eliminated. Cell cultures not only provide means for de novo synthesis of natural products but also serve for bioconversion of low value compounds into high value products. Taking in account that wild Opuntia species are widely distributed along the semiarid regions of Mexico where hard environmental conditions prevail, they must express a higher level of metabolites than domesticated species O. ficus indica. For that reason, we have the main goal to characterize the metabolites and proteins profile from wild Opuntia species where at least five levels of domestication are found. To avoid variations due to different environmental conditions and/or culture conditions, the plants will be collected from the CHAPINGO University/ Agriculture, localized at Orito municipality, Zacatecas state, México. With the used of novel technologies for the study of plant metabolomics and proteomics, at least ten compounds will be tested on several biological/pathological end-points (colon cancer, atherosclerosis, obesity). With the results of the present proposal we expect to generate novel molecular information that could give to Opuntia species an increased biotechnological value. This information will help to protect the wild species and to developed strategies to produce metabolites with high potential, as new bioactive compounds against several illnesses. In addition, the comparison of protein profile of wild plants and domesticated species give insights about the mechanisms of phytochemical biosynthesis.
Madame Françoise GUÉRAUD (INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE -CENTRE DE RECHERCHE DE TOULOUSE) – email@example.com
The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.
UASLP UASLP, Mexique
UASLP UASLP Zonas deserticas, Mexique
IPICYT IPCYT, Division de Biologia Molecular
IPBS CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - DELEGATION REGIONALE MIDI-PYRENEES
INRA INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE -CENTRE DE RECHERCHE DE TOULOUSE
INSERM INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE - Délégation régionale Midi-Pyrénées Limousin
Help of the ANR 227,394 euros
Beginning and duration of the scientific project: - 36 Months