Cryo-electron microscopy (cryo-EM) is under constant development for a routine determination of biomolecular structures (conformations) at high resolution. This project is focused on the development of approaches for identifying continuous conformational transitions from cryo-EM images through high-resolution determination of the entire conformational variability of studied complexes. We have established the basis for one such approach by combining image analysis with molecular mechanics simulations. To obtain high-resolution information regarding the full conformational variability, three simulation methods will be studied in combination with image analysis and artificial intelligence methods. The new approaches will be useful for studying a large range of biomacromolecular complexes in the field of structural biology. Synthetic cryo-EM data of various complexes will be used to validate and compare these approaches. The best-performing approach will be valorized using experimental cryo-EM data of two biomedically important complexes (human 80S ribosome and human p97 AAA ATPase).
Madame Slavica Jonic (Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie)
The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.
Nagoya University / Department of Physics and ITbM
IMPMC Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie
IGBMC INSTITUT DE GENETIQUE ET DE BIOLOGIE MOLECULAIRE ET CELLULAIRE
University of Melbourne / Department of Biochemistry and Molecular Biology/ Bio21
Help of the ANR 505,558 euros
Beginning and duration of the scientific project: September 2019 - 48 Months