A novel nicotianamine-based metallophore in pathogenic bacteria – ANIBAL
Ensuring the availability of a given metal for growth is a vital microbial process, especially critical when the source becomes scarce like inside a host. Here, we present a project that is dedicated to the study of an operon expected to encode the different functions required for the synthesis, export and recovery of a novel bacterial metallophore analogous to plant nicotianamine. Preliminary studies indicate that this system is functional, dedicated to the import of nickel and cobalt, and that the transporters are important for the virulence of S. aureus. Our objective is to study this novel metallophore in S. aureus and P. aeruginosa through biochemical, genetic and metabolomics approaches. We will determine the structure of the metallophore, study the mechanism and regulation of this system, define the biochemical and structural basis of the biosynthetic machinery and finally determine the importance of the metallophore synthesis in the virulence of S. aureus and P. aeruginosa.
Monsieur Pascal ARNOUX (Laboratoire de Bioénergétique Cellulaire)
The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.
LCABIE-IPREM Institut des Sciences Analytiques et de Physicochimie pour l'Environnement et les Matériaux
CEA-LBC Laboratoire de Bioénergétique Cellulaire
CNRS DR12 CNRS DR PROVENCE ET CORSE
Help of the ANR 449,907 euros
Beginning and duration of the scientific project: September 2014 - 36 Months