RA-COVID-19 V11 - Recherche - Action Coronavirus disease 2019 - Vague 11

Functional Interrogation of SARS-CoV-2 RNA-Host Binding Proteins in viral replication – FISHBP

Submission summary

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 pandemic, which over a span of few month, has caused a devastating global health crisis. In absence of antiviral therapies or approved vaccine, there is an urgent need to understand the molecular interaction that the virus establishes with the cell host during infection to identify new therapeutic targets.
SARS-CoV-2 is a positive RNA virus that relies on proteins and biological pathways of the host cell to accomplish its lifecycle. This host dependence represents an “Achilles heel” that could be exploited to develop new approaches to treat SARS-CoV-2. To uncover cell host factors that regulate viral infection, we used a comprehensive identification of RNA binding proteins by mass spectrometry (ChIRP-M/S) approach and identified 142 high-confidence cell factors that associate with SARS-CoV-2 genomic RNA in infected cells. The global aim of the FISHBP proposal is to decipher the function of these proteins during the SARS-CoV-2 life cycle by combining systematic functional screens and hypothesis-driven approaches.
In a year time, this proposal will strengthen our comprehensive view of host protein-SARS-CoV-2 genomic RNA interactome involved in viral replication by extending our understanding of how these host factors modulate viral replication. It will provide a valuable source of information for future studies aiming to understand the molecular mechanism of viral pathogenesis and/or to develop new antiviral therapeutics.

Project coordinator

Monsieur Laurent Meertens (Génomes, biologie cellulaire et thérapeutiques / GENomesand CELL biology Diseases)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

GenCellDi Génomes, biologie cellulaire et thérapeutiques / GENomesand CELL biology Diseases

Help of the ANR 100,980 euros
Beginning and duration of the scientific project: January 2021 - 12 Months

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