REgulators of Membrane INteracting Domains in Toxoplasma gondii – REMIND
Apicomplexa include life-threatening intracellular parasites such as those causing malaria and toxoplasmosis. These parasites have unique secretory organelles that are essential for host infection. Our knowledge of the biochemical mechanisms underlying the biogenesis of such organelles remains quite incomplete, hindering our understanding of apicomplexan infection. We have discovered a novel parasite-specific protein, TgREMIND (Toxoplasma gondii REgulators of Membrane INteracting Domains) that contains both membrane lipid and Rab-binding domains, along with a novel domain whose functions are unknown. In this project, we will combine biochemistry, structural biology, reconstitution assays with membrane models, cell biology and reverse genetics in order to decipher TgREMIND functions. We envision using TgREMIND and two other related proteins as proofs of concept that parasite-specific proteins can finely regulate the protein/lipid trafficking required for secretory organelle biogenesis.
Monsieur Stanislas TOMAVO (CNRS UMR 9198)
The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.
I2BC CNRS UMR 9198
IPMC CNRS UMR 7275
I2BC Institut de Biologie Intégrative de la Cellule
Help of the ANR 511,626 euros
Beginning and duration of the scientific project: September 2019 - 48 Months