DS0304 -

Development and Optimization of Production process of Extremely Oxygen Sensitive bacteria for their industrial exploitation – DOPEOS

Submission summary

Many bacteria on earth are obligate anaerobes that do not grow because of their extreme sensitivity to oxygen (EOS) and of specific requirement for culture. Despite their crucial roles and their strong functional potential, these bacteria are not yet well exploited source in the areas of food, well-being and health because of their growth requirements. One major challenge is to anticipate the future use such beneficial EOS bacteria by preparing their large-scale production. This industrial production capacity, currently uncontrolled, is the main obstacle to the use of these microorganisms for which culture is starting to be controlled at small scale in specialized laboratories. The overall objective of DOPEOS project is to define conditions for biomass production and preservation of two intestinal EOS bacteria that are archetypes of beneficial microbes with high economic potential. This project will lead to the design of a specific bioreactor for the production of EOS bacteria and to its integration in an industrial production line of microbial biomass. To achieve these objectives, DOPEOS brings together a consortium of two academic teams (UMR PAM and Micalis) and two French SMEs (Biovitis, specialized in the production of biomass and Global Process Concept (GPC), specialized in the design of production lines).

The scientific objectives of DOPEOS are to identify the specific conditions (substrates, gas...) necessary for the growth of bacteria and to decipher the relationships between oxygen and their(s) activity(ies) or survival. These laboratory studies will lead to pilot tests on realistic production line (Biovitis) and to the development of a strategy and a process for producing EOS bacteria at large scale in a specific bioreactor (GPC). DOPEOS also includes the search for a stabilization and preservation process (post-production of biomass) in order to design a complete production line (GPC), integrating the bio-reactor in the chain of unit operations (fermentation, centrifugation, dehydration, packaging). Experiments at lab scale will take place within the UMR PAM, laboratory recognized as expert for the study of the influence of environmental conditions on survival and activities of microorganisms and for the design of experimental reactors. Quality of microorganisms at different scales will be evaluated by measuring their functionality. This is an original approach that places the functional preservation at the heart of the design of the industrial process for production of EOS bacteria. The evaluation of the functional preservation of the bacteria will be carried out by UMR Micalis, international expert in the field of intestinal health / food and health interest bacteria. The design of the specific bio-reactor for the production of EOS bacteria will take place following a scaling-up process in which the laboratory data will be transposed on a modified production line (Biovitis). These pilot tests will help to define the specifications required for the design and conception (GPC) of the dedicated bio-reactor for EOS bacteria. The implementation of the bio-reactor and the plans for its integration into a production line will be provided by GPC on the basis of data from laboratory and pilot experiments, and on our own design expertise.

DOPEOS is organized to promote interactions between partners, a necessary condition for progression on the three scales (laboratory, pilot and industrial). Our ambition is to produce scientific and technological knowledge for offering a vanguard solution with high economic potential. DOPEOS will involve about 25 persons for 3 years.

Project coordination

Laurent Beney (UMR Procédés Alimentaires et Microbiologiques)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.


UMR PAM UNIV DIJON UMR Procédés Alimentaires et Microbiologiques
GPC Global Process Concept

Help of the ANR 639,770 euros
Beginning and duration of the scientific project: December 2016 - 36 Months

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