gene targeting; genetically modified crops; meganuclease; TALEN; CRISPR-Cas9; recalcitrant; elite lines; in planta; plant breeding; socio-economic impact

Ingénierie cellulaire : amélioration et innovation technologiques pour les plantes d'une agriculture

Biotech - Bioressources (BTBR)

Biotech - Bioressources

Informations générales

Référence projet : 11-BTBR-0001
RST : Peter ROGOWSKY
Etablissement Coordinateur : INRA Saint Genes Champanelle
Région du projet : Auvergne-Rhône-Alpes
Discipline : 4 - Agro Eco

Aide de l'ANR 6 000 000 euros
Investissement couvrant la période de août 2012 à juin 2020

Résumé de soumission

GENIUS aims to provide to French researchers and plant breeders state of the art genome editing tools in a variety of crop species..

2012 Major event: Kick-off meeting

2013 Major events: Release of communication tools, first philosophical debate. Results: Design and production of site directed nucleases, choice of amiRNA backbones and production of respective constructs. First improvement of transformation rates of recalcitrant species (rose, apple).

2014 Major events: Meeting with scientific advisory board, project poster, signature of consortium agreement, second philosophical debate. Results: Production of stable transformants for nucleases, reporter constructs and landing pads. First evidence for nuclease-based deletion in tomato. Massive sequencing of land pad flanking sequences in rice and wheat. DNA cleavage (low efficiency) by a nuclease delivered as protein in moss. Improved transformation frequencies of certain elite lines (apple, rose).

2015 Major Events: Study day on ethics and food. Results: Demonstration of RAG1 activity in tomato. Functional NptII TALEN® in wheat open the way for gene targeting. Validation of miRNA backbones for gene extinction in rose and apple. Successful gene knock-out in rice (CRISPR/Cas9) and gene knock-in (gene editing) in tomato (TALEN®). Further optimization of rose regeneration, of rose and apple transformation and of protoplast regeneration in two elite potato varieties.

2016 Major Events: Departure of partner Cellectis, meeting with scientific advisory board, reorientation of work program, debate on urban food production, mapping of biotech projects. Results: Targeted DNA cleavage by RAG1 or I-SceI demonstrated in 5 crop species, landing pads established in 7 crop species. CRISPR-Cas9 mediated targeted mutations achieved in rice, maize and potato and CRISPR-Cas9 mediated gene editing in tomato. Delivery of CRISPR-Cas9 as ribonucleoprotein (RNP) complex in Physcomitrella. In planta transformation established for poplar.

L'auteur de ce résumé est le coordinateur du projet, qui est responsable du contenu de ce résumé. L'ANR décline par conséquent toute responsabilité quant à son contenu.

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