Network of regulatory RNAs across kingdoms and dynamical responses to biotic and abiotic stresses.

The LabEx NetRNA coordinated by P. Romby (since sept 2016), included 12 teams in 2018. The general objective is to advance knowledge on the functions of novel RNAs, their targets and mechanisms in a variety of pathogens and of organisms (plants, insects, and mammals). NetRNA focuses on regulatory RNAs, and their machineries that play a role in development, stress-related responses, immune responses, host-pathogen interactions, and in human diseases. Important findings have been obtained on the machineries assisting and controlling regulatory RNAs in plants, insects and various pathogens (bacteria, viruses, parasites), and on their impact on genome surveillance, translational, post-transcriptional and post-translational controls. These data were accompanied by the development of novel methodologies: (1) Mass spectrometry identified protein partners and protein interactome from complex samples, and modifications of proteins and RNAs; (2) droplet-based microfluidics led to novel fluorescent probes targeted to RNAs; (3) Next-generation sequencing approaches designed to quantify small non-coding RNAs and analyze RNA modifications. The junior chair headed by JT Marques has started in spring 2018 to study the control of arboviruses by mosquito antiviral pathways. One of the junior teams has been awarded an ERC consolidator grant. For scientific animation, we have invited several renowned international researchers and have provided support for the organization of mini-symposium in Immunology and of a new UE in ethics for Master students (Master of Developmental biology, Molecular Biology). The international RNA society provided financial support to create the StrasRNA salon favoring year-long interaction and sharing of knowledge amongst RNA researchers. Finally, NetRNA is a partner of the Graduate School “Integrative Molecular and Cellular Biology ” which has been selected in 2017. All these aspects contributed to promote a top-level training for young researchers.