Characterization of the role of betacoronavirus M protein in viral assembly – coronem
Coronaviruses are positive-stranded RNA, enveloped viruses. The lipidic envelope contains 3 viral proteins with the membrane protein (M) being the most abundant component and the driving force in viral assembly. The main objective of this project is to better understand the morphogenesis of MERS-CoV and SARS-CoV-2 by focusing on the role of the M protein in this process. More precisely, we will identify the M protein regions(s) involved in viral assembly and determine their mechanism of action. In our approach, the identified regions will be assessed for their function in the intracellular trafficking of the protein to the budding site and in interaction with the other viral proteins, keeping in mind that the intracellular trafficking of the MERS-M protein may affect protein-protein interaction and conversely. We also aim at determining the structure of the M protein and at identifying cellular factors involved in coronavirus assembly and how the M protein may recruit these factors.
Madame Sandrine Belouzard (Institut Pasteur de Lille - CIIL - Virologie Moléculaire et Cellulaire)
The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.
IPL-CIIL-VMC Institut Pasteur de Lille - CIIL - Virologie Moléculaire et Cellulaire
MAVIVH Université de Tours
IPL-BSI Institut Pasteur de Lille - Biologie structurale intégrative
Help of the ANR 388,093 euros
Beginning and duration of the scientific project: September 2022 - 36 Months