CE20 - Biologie des animaux, des organismes photosynthétiques et des microorganismes

Function of chloroplast nucleoids in C4 photosynthetic differentiation. – TOCCATA

Submission summary

In C4-plants the separation of photosynthetic functions between highly specialized mesophyll (M) and bundle sheath (BS) chloroplasts allows an efficient CO2 fixation. Gene networks at the origin of the C4-syndrome remain largely undiscovered particularly at the level of plastid gene expression (PGE). Considering high differentiation of the photosynthetic apparatus in M and BS chloroplasts, we hypothesize that regulatory factors responsible of C4-specific PGE will act at the level of the nucleoids (plastid protein-DNA-RNA complexes) that regroup proteins implicated in all levels of PGE. Our proposal aims at revealing C4-specific regulations in a comparative and never undertaken approach combining proteomics and transcriptomics of M and BS nucleoids. This parallel aims to reveal proteins and post-transcriptional mRNA maturation events implicated in C4-diferentiation. Functions and mechanisms of action of candidate proteins will be explored through reverse genetic analysis.

Project coordinator

Monsieur Wojciech Majeran (Université Paris-Saclay / Institut des Sciences des Plantes de Paris Saclay)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

INRAE / IPS2 Institut national de recherche pour l’agriculture, l’alimentation et l’environnement / Institut des Sciences des Plantes de Paris Saclay
UPSaclay / IPS2 Université Paris-Saclay / Institut des Sciences des Plantes de Paris Saclay
Cornell University / Chloroplast biology van Wijk lab.

Help of the ANR 414,725 euros
Beginning and duration of the scientific project: March 2022 - 48 Months

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