CE20 - Biologie des animaux, des organismes photosynthétiques et des microorganismes

Advanced METallophore characterization by HYphenated techniques in the Soil-microorganisms-plants Ternary Environmental Systems – AMETHYSTES

Submission summary

Improving yield and nutrient content of crop plants is a critical issue as deficiencies are very common in populations where plant-based nutrition is predominant. An effective strategy followed by microorganisms and plants is to release specific chelating molecules, which complexes metal ions and makes them soluble.
Until recently, the molecules produced by microorganisms to bind metal in their environment were essentially considered as siderophores (iron(III) scavengers). However, latest studies, included from applicants, revealed that, other metal ions than Fe(III) are targeted and that a significant fraction of non-Fe metal ions were bound to these chelating molecules. The general term of “metallophore” is therefore more appropriate. As metallophore cocktails produced by a given strain or microbiota are usually poorly characterised, there is a critical need to develop proper methodologies to follow the fate of all the metallophores in environmental sample and to discover their essential roles in valuing beneficial associations between plants and microorganisms.
The objective of this project is to develop a systematic and essentially mass spectrometry-based analytical methodology to have an efficient tool to better screen the environmental and biological roles of metallophores in the interaction between rhizosphere microbiotas and cultivated plants. During this project, applicants will characterize the presence of all the potential metallophores in samples from bacterial and plant investigations (extract, past culture media, sap, collected tissues…). They will set up experimental designs that will allow answering questions about the uptake of metal-metallophores complexes by plants. To be able to track precisely the metallophore and obtain analytical proof of its uptake, participants are aiming at a screening of metallophores by molecular high-resolution mass spectrometry using appropriate sample preparation/separation techniques and methodology based on metal-metallophore complexes enriched in stable isotopes.
The project will focus on the cell culture of microorganisms of interest and on culture conditions to reveal metallophore-based metal binding strategy developed by rhizosphere microorganisms. Participants will study especially key bacterial groups, like Pseudomonas, and synthetic community inspired by microbiota composition. In addition, experiments with selected bacteria of interest and model plants will help to better understand their environmental and biological roles (symbiosis, competition ...) in soil-microorganism-plant systems. For this purpose, applicants will firstly assess the impact of bacterial metallophores on metal homeostasis in plants, in particular their ability to relieve metal deficiency symptoms and to be transported up to shoots. The study will be conducted in parallel using two model species, a higher plant (Arabidopsis thaliana) and a primitive plant (a moss, Physcomitrella patens) to compare evolutionarily-distant plants, and using two crops, pea (Pisum sativum) and wheat (Triticum aestivum) that have different iron acquisition strategies.
On the long term, the type of knowledge collected during this project should help to improve the essential element uptake (Fe, Zn, Mn…), growth in metal depleted soils and resistance to phytopathogens of agricultural plants.

Project coordinator

Monsieur Laurent OUERDANE (INSTITUT DES SCIENCES ANALYTIQUES ET DE PHYSICO-CHIMIE POUR L'ENVIRONNEMENT ET LES MATERIAUX)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

AGROECO. AGROECOLOGIE - UMR 1347
IPREM INSTITUT DES SCIENCES ANALYTIQUES ET DE PHYSICO-CHIMIE POUR L'ENVIRONNEMENT ET LES MATERIAUX
BPMP Biochimie et Physiologie Moléculaire des Plantes

Help of the ANR 561,969 euros
Beginning and duration of the scientific project: December 2021 - 48 Months

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