CE20 - Biologie des animaux, des organismes photosynthétiques et des microorganismes

RNase J and chloroplast RNA Quality Control – JOAQUIN

Submission summary

The chloroplast is the plant compartment in which light energy is captured and transformed into chemical energy, ultimately making life on Earth possible. They are descendants of free-living photosynthetic bacteria and maintain a small but essential genome. Most genes are transcribed into polycistrons that are then heavily processed by a combination of RNA-binding proteins and ribonucleases to produce the functional RNA population. One poorly understood aspect of chloroplast gene expression and regulation is the functions of asRNAs and more globally double-stranded RNA-related processes. One of the key enzymes in these processes is RNase J, whose characteristic is to be both a 5’-3’ exoribonuclease and an endoribonuclease. It trims RNA termini 5’ ends and eliminate deleterious antisense RNA that could harm translation. The goal of the JOAQUIN project is to gain a better understanding of chloroplast RNA quality control by (i) identifying the full set of chloroplast RNA isoforms and RNA-RNA duplexes and (ii) and understand how this transcriptome is shaped by RNase J. The substrate specificity of RNase J will be deciphered focusing on the land plant’s specific C-terminal GT-1 domain and by the identification of the protein and nucleic acids that co-purify with the enzyme.

Project coordination

Benoit Castandet (Université Paris-Salay - Institut des Sciences des Plantes de Paris Saclay)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

UPSaclay - IPS2 Université Paris-Salay - Institut des Sciences des Plantes de Paris Saclay

Help of the ANR 222,472 euros
Beginning and duration of the scientific project: February 2021 - 48 Months

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