INNOVATIVE NANOCOMPOSITES RELEASING BIOLOGICAL FACTORS BY MAGNETIC HYPERTHERMIA AS COMPONENTS OF SMART SCAFFOLDS FOR TISSUE ENGINEERING – CORELMAG
A main challenge in the field of Tissue Engineering (TE) is the design of smart scaffolds releasing remotely bioactive proteins such as growth factors (GFs) by externally applied stimuli (e.g., light or magnetic waves).
In the CORELMAG project, we propose at first to design original multifunctional nanocomposites (MFNCs) composed of a magnetic core optimized for magnetic hyperthermia (MH) and surrounded with a stellate mesoporous silica shell having large pores ensuring the suitable loading and magnetothermal release of GFs. Then, such MFNCs will be integrated in electrospun (ELS) scaffold to form a smart scaffold displaying a magnetically induced delivery of GFs for TE. While the design of such ELS is well mastered by my collaborators, the design of MFNCs face different key challenges.
A first challenge consists in controlling the local surface T of the nanocomposite to ensure GFs release while avoiding its denaturation. This will be achieved by a nanothermometry study under alternating magnetic field and by a theoretical modelling study to better understand the thermal transfer. A chemical engineering of the MFNCs will allow thus to adjust precisely the local T.
Another challenge is the thermoresponsive interaction between MFNCs and GFs and we will develop a promising and innovative strategy. Indeed this novel thermoresponsive interface would allow loading large amount of GFs and also triggering their release under local heat. The proof of concept that these thermoresponsive groups allow protein release at moderate T (42-50°C) was shown recently and will be optimized in the project.
Thus, innovative MFNCs loaded with high amount of Vascular Endothelial Growth Factor (VEGF), a well-established GF, will be thus designed and will be assessed for magnetothermal desorption. These smart responsive nanocomposites will be then integrated/formulated within a polymer scaffold elaborated by electrospinning method. The scaffold structure allowing to release magnetothermally the highest amount of VEGFs will be evaluated and finally a preliminary biological study will be conducted to assess the delivery on endothelial cells which can promote angiogenesis and finally tissue integration and regeneration.
Monsieur Mertz Damien (Institut de physique et chimie des matériaux de Strasbourg (UMR 7504))
The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.
IPCMS Institut de physique et chimie des matériaux de Strasbourg (UMR 7504)
Help of the ANR 198,713 euros
Beginning and duration of the scientific project: September 2019 - 42 Months