CE44 - Biochimie du Vivant

High performance MS-based proteomics by reducing stable isotopes complexity in vivo – SLIM-labeling

Submission summary

One of the most challenging problems in modern biology is to address at the experimental level the analysis of the dynamics of proteome variations in composition and structure in order to decipher the fundamental mechanisms of gene expression and regulation in normal and pathological conditions. Using U-[12C]-glucose as the sole source of carbon to grow prototrophic cells, we developed a Simple Light Isotope Metabolic (SLIM) labeling strategy highly effective to analyze with an unprecedented depth complex proteomes in bottom-up and top-down experiments. We want now to elaborate an automatic workflow processing of MS bottom-up raw data for quantitative proteomics. This requires robust statistical analysis of the SLIM-labeling based quantitative proteomics procedures. We want to apply the SLIM-labeling strategy to analyze the quantitative variations of proteoforms in top-down experiments, addressing the complexity of proteome from multi-cellular organisms and higher eukaryote cells.

Project coordinator

Monsieur jean Michel Camadro (Institut Jacques Monod)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.


CNRS - I2BC Institut de Biologie Intégrative de la Cellule
NCBI - QMBP National Center For Biotechnology Information / Quantitative Molecular Biological Physics
IJM Institut Jacques Monod
IJM Institut Jacques Monod

Help of the ANR 285,276 euros
Beginning and duration of the scientific project: September 2018 - 36 Months

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