CE42 - Capteurs, instrumentation

Odorant Binding Proteins-based biomimetic optical nose – OBP-optinose

OBP-optinose

Odorant Binding Proteins-Based Biomimetic Optical Nose

Development of new sensitive materials to significantly improve the performance of electronic noses

Today, there is a growing demand for sensitive and selective detection of volatile organic compounds (VOCs) in various domains such as environment monitoring, public safety, quality control, non-invasive medical diagnostics. Traditional methods, though accurate and reliable, require expensive equipment and are often time-consuming and laborious. In this context, electronic noses (eNs) have emerged as promising tools for analysis of VOCs. However, so far, the performance of existing eNs is still far behind that of human nose, which relates primarily to the sensing elements. Hence, we propose to develop a new biomimetic optoelectronic nose with the aim to improve greatly eN performances (sensitivity, selectivity and specificity).

We propose to design and prepare novel sensing materials by mimicking human nose with the combination of specific and cross-reactive recognition principle. We will use wild-type odorant binding proteins (OBPs) as “broadly tuned” sensing materials, with cross-reactivity to VOCs. Moreover, based on molecular modeling and protein engineering, we will design and produce a set of “narrowly tuned” OBPs and more stable OBP alternatives with high specificity for some target VOCs. All cross-reactive and specific OBPs will be integrated and arrayed on the same chip and then coupled with the handheld NeOse developed by Aryballe Technologies.

With the help of molecular docking, 6 specific OBPs were designed. They were successfully produced together with 7 cross-reactive OBPs in this project. All of them were immobilized on the chip for the development of novel eN. OBP chip fabrication was optimized and a reproducible procedure was established. A particular effort was made to investigate the effect of humidity on the performances of the obtained eN. It was found that the humidity is essential for conserving activities of OBPs. The obtained eN has very good sensitivity and selectivity for target VOCs. After integrating the OBP chip into miniaturized SPRi NeOse, the portable eN conserved these performances.

This new eN is complementary to the generic approach employed currently by Aryballe. It may allow the company strengthening present applications and more importantly targeting new markets such as the detection of olfactory pollution, already identified by Aryballe as one of the most attractive markets.

1. Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review, C. Hurot, N. Scaramozzino, A. Buhot, Y. Hou, Sensors, 2020, 20, 1803.
2. Bacterial expression and purification of vertebrate odorant-binding proteins, Marine Brulé, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers, Loïc Briand, Methods in Enzymology, 2020, 642, 125.
3. Ligand binding properties of odorant-binding protein OBP5 from Mus musculus, L. Moitrier, C. Belloir, M. Lalis, Y. Hou, J. Topin, L. Briand, Biology, 2023, 12, 2.
4. An Overview of Olfactory Sensors and Electronic Noses Based on Surface Plasmon Resonance for Analysis of Volatile Organic Compounds, M. El kazzy, J. S. Weerakkody, C. Hurot, R. Mathey, A. Buhot, N. Scaramozzino and Y. Hou, Biosensors, 2021, 11(8), 244.
5. Biomimetic olfactory biosensors and bioelectronic noses, M. El Kazzy, C. Hurot, Jonathan S. Weerakkody, A. Buhot, Y. Hou, Advances in Biosensors: Reviews, Book Series, Vol. 3, 2020, Chapter 1, 15-63.
6. Odorant binding protein-based optoelectronic nose: Hydration and protein activity, M. El kazzy, C. Hurot, A. Buhot, L. Moitrier, C. Belloir, L. Briand, Y. Hou, 2022 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), page 1-3. (DOI: 10.1109/ISOEN54820.2022.9789645)

Today, there is a growing demand for sensitive and selective detection of volatile organic compounds (VOCs) in various domains such as environment monitoring, public safety, quality control, non-invasive medical diagnostics. Traditional methods, though accurate and reliable, require expensive equipment and are often time-consuming and laborious. In this context, electronic noses (eNs) have emerged as promising tools for analysis of VOCs. However, so far, the performance of existing eNs is still far behind that of human nose, which relates primarily to the sensing elements. Hence, we propose to develop a new biomimetic optoelectronic nose with the aim to improve greatly eN performances (sensitivity, selectivity and specificity).
For this, we will design and prepare novel sensing materials by mimicking human nose with the combination of specific and cross-reactive recognition principle. We will use wild-type odorant binding proteins (OBPs) as “broadly tuned” sensing materials, with cross-reactivity to VOCs. Moreover, based on molecular modeling and protein engineering, we will design and produce a set of “narrowly tuned” OBPs and more stable OBP alternatives with high specificity for some target VOCs. All cross-reactive and specific OBPs will be integrated and arrayed on the same chip and then coupled with the handheld NeOse developed by Aryballe Technologies. Such eN is complementary to the generic approach employed currently by Aryballe. It may allow the company strengthening present applications and more importantly targeting new markets such as the detection of olfactory pollution, already identified by Aryballe as one of the most attractive markets.

Project coordinator

Madame Yanxia HOU-BROUTIN (Systèmes Moléculaires et nano Matériaux pour l'Energie et la Santé)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

ARYBALLE TECHNOLOGIES
CSGA CENTRE DES SCIENCES DU GOUT ET DE L'ALIMENTATION
SyMMES Systèmes Moléculaires et nano Matériaux pour l'Energie et la Santé
UNS - ICN Université Nice Sophia Antipolis - Institut de Chimie de Nice

Help of the ANR 460,612 euros
Beginning and duration of the scientific project: February 2019 - 36 Months

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