TERC - Tremplin-ERC

Multiplexed diagnostics of microRNA using ultra-sensitive homogeneous FRET detection – amplify

Submission summary

The amplify project aims at the development and application of a new diagnostic tool for faster and better detection of microRNA (miRNA) cancer biomarkers. The technology will combine ultra-high sensitivity and multiplexing of several different miRNAs in a one-step-preparation and rapid-detection, homogeneous assay format. Both the detection of circulating miRNA (plasma) and miRNA in-situ hybridization on the single-cell level (cells, tissues) and in living cells will be demonstrated. Direct benefit for cancer diagnostics will be evidenced on clinical samples from cancer patients and healthy individuals and the results will be compared to state-of-the-art miRNA detection technologies. amplify will develop three major cornerstones: i) highly specific (distinction of single base pair mismatches) and highly sensitive (sub-femtomolar) homogeneous detection of endogenous miRNAs in plasma; ii) multiplexed detection of endogenous miRNAs from a single plasma sample; iii) high-order multiplexed miRNA in-situ hybridization imaging.
The frontier research concept of amplify is to combine the power of isothermal nucleic acid amplification for ultra-sensitive detection with the versatility of FRET for multiplexed and homogeneous (separation-free) optical biosensing. Direct quantification of multiple different miRNAs from a single human plasma sample, without laborious preparation and separation steps and the need for sophisticated equipment and analysis, has the potential for a paradigm shift in clinical diagnostics of miRNA, because it combines the simplicity of a homogeneous immunoassay, the multiplexing capability of microarrays, the sensitivity of qRT-PCR, and the throughput capacity of next generation sequencing within one single diagnostic tool. The implementation of the same technology into in-situ hybridization in cells and tissues will present a breakthrough in multiplexed detection, which is currently limited to approximately three colors.

Project coordination

Niko Hildebrandt (Institut de Biologie Intégrative de la Cellule)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

I2BC Institut de Biologie Intégrative de la Cellule

Help of the ANR 217,080 euros
Beginning and duration of the scientific project: April 2017 - 18 Months

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