Capillary supercritical fluid chromatography coupled to mass spectrometry for the analysis of biomolecules – Cap-SFC-MS

Taking into account growing public concerns on environmental questions, analytical studies and the consequent use of toxic solvents have increased to such a level that it becomes untenable to continue without changing towards environmentally friendly methods. Supercritical Fluid Chromatography (SFC) coupled with mass spectrometry (MS) is one solution for the present and the future. Nevertheless some limitations need to be broken to make SFC-MS popular in “omics” world. The project is based on the development of a new capillary packed column SFC platform for low volume and highly diluted biosamples. Fundamental and practical questions on the coupling with MS will be addressed in order to optimize sensitivity and structural characterization of natural products. Method developments in proteomics, lipidomics and epigenetics are also proposed taking advantages of the newly developed instrument.