DS0410 -

Physiopathology of the ubiquitine Ligase E3, PDZRN3, in the development of eccentric hypertrophy and transition to heart failure – CardioPDZ

Submission summary

Left ventricle hypertrophy (LVH) is an adaptive response of the heart to an increase workload. At the beginning, LVH is beneficial to maintain cardiac output by reducing wall stress, but it becomes maladaptive in the chronic phase, resulting in heart failure (HF). HF remains a deadly syndrome with 5-year mortality of 40-60%. Advancement of the understanding of the mechanisms underlying LV remodeling and the transition to HF is a prerequisite for the development of new efficient management strategies.
A prominent and unique feature of cardiac muscle is the presence of intercalated discs (ID) at polarized ends of cardiomyocytes. ID are the “glue” which ensures mechanical and electrical coupling from one cardiomyocyte to another. Soon after birth, the cardiomyocytes elongate, ID are organizing toward a mature state. Human studies have shown that during LVH process, hypertrophied myocytes harbor dedifferentiated phenotype (ID destructuration, sarcomere depletion, glycogen accumulation, and alteration of mitochondria). It is proposed that these changes toward a fetal-like phenotype represent an adaptive programmed cell survival response. However, the biology of this phenomenon of recapitulation of an immature phenotype of the failing myocardium is largely unknown.
The global objective of this proposal is to decipher new molecular mechanisms driving the LV remodeling and the transition to failure. Wnt/Planar Cell Polarity (PCP) pathway has recently emerged as a major regulator in adult organs for tissue morphogenesis. This ubiquitous system coordinates cellular communications; it involves polarized coupling between adjacent cells. By applying PCP features to heart morphogenesis, we hypothesize that the Wnt/PCP pathway may signal in the cardiomyocytes and control their polar organization and their remodeling and in fine the molecular balance from dedifferentiated immature toward a differentiated a phenotype and vice versa. Our working proposed model is that Wnt/PCP signaling is required during fetal maturation, for the organization of cardiomyocytes (immature phenotype) and is repressed when cardiomyocytes undergo differentiation

In this project, we have asked whether Wnt/PCP signaling is reactivated during hypertrophic cardiomyopathy development and involved in HF. Our objective is to decrypt PCP signaling and its role in the process of cardiac morphogenesis. It will be the first demonstration that PCP signaling is involved in the physiopathology of the transition from LVH to HF.

The core molecular machinery and regulation of PCP pathway is far to be known in mammals. We have recently identified a novel actor of PCP pathway: the ubiquitine E3 ligase, PDZRN3 and demonstrated its upstream critical role in the regulation of the Wnt/PCP signaling. To tackle this project, we have then developed original tools to overexpress and to delete specifically Pdzrn3 in cardiomyocytes in vivo (transgenic mice) and in vitro. In order to better understand the physiopathology basis of this reported phenotype of Pdzrn3 mutant mice, we have collected cardiac human tissues from patients suffering from various cardiomyopathies.

Utilization of animal and cellular models to probe causes and mechanisms of cardiomyopathy disease will result in breakthroughs in diagnosis or prognosis tools or in development of novel treatments that will hopefully improve the outcome for HF patients.

Project coordinator

Madame Cécile DUPLAA (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

UMRS 1166 Unité de recherche sur les maladies cardiovasculaires, le métabolisme et la nutrition
INSERM INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE

Help of the ANR 403,745 euros
Beginning and duration of the scientific project: October 2016 - 36 Months

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