DS0401 -

Bacillus subtilis Regulatory RNAs and mRNA degradation – BaRR

Submission summary

Post-transcriptional regulation is a highly efficient way to modulate gene expression used by all organisms. Small regulatory RNAs (sRNAs) play a key role in this mode of control and can directly affect both mRNA translation and degradation. sRNAs have been primarily studied in Gram-negative bacteria and in some Gram-positive pathogens e.g. Staphylococcus aureus. However, in Bacillus subtilis, the paradigm of Gram-positive bacteria naturally present in soil, only a handful of sRNAs have been characterized to date and the mechanisms of regulation by sRNAs have not been deciphered. From previous studies, it is clear that regulation by sRNAs in the Firmicutes works differently. For example, in contrast to E. coli, sRNAs do not need the Hfq protein to foster sRNA-mRNA interactions. Moreover, there are major differences between the Gram-negative and Gram-positive mRNA degradation machineries. Indeed, RNase E is the main enzyme involved in degradation of mRNA after sRNA base-pairing in E. coli, but this RNase is absent in B. subtilis. We propose to measure the impact of sRNA on translation and mRNA degradation in B. subtilis and identify RNases involved in this regulation. We also dedicated a part of the project to the identification of protein partners of sRNA-mRNA regulations since no functional homolog of Hfq has been identified in B. subtilis. From these perspectives, the main aim of this project is to get a better understanding of post-transcriptional regulation by sRNAs (e.g. RoxS, FsrA and CsfG) in the model organism B. subtilis.

Project coordination

Sylvain DURAND (Expression génétique microbienne)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

CNRS - UMR8261 Expression génétique microbienne

Help of the ANR 229,495 euros
Beginning and duration of the scientific project: - 36 Months

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