DS0413 - Technologies pour la santé

MOLecular endoscopY for remote in vivo diagnosis – MOLY

Submission summary

Our objective is to design in this three-year project an original “lab-on-fiber” tool for remote, label-free “in vivo” molecular analysis that could be dedicated in the future to endoscopic diagnosis. While micro-arrays are currently used “in vitro” for multi-parametric detections of biomarkers, concerning “in vivo” molecular detection, no multi-parametric label-free approach has been developed so far. Nonetheless this tool could be very useful and bring significant progress in the medical field especially in diagnosis technique like endoscopy. This tool could efficiently complete this imaging medical analysis. Indeed, the diagnosis would be facilitated by the real time detection, quantification, and localization of specific pathologies’ biomarkers. This tool-associated gain could highly benefit to the patients and significantly reduce the overall cost of diagnosis.
To achieve this project our approach is based on functionalized nanotextured optical fiber bundles. When appropriately designed and covered by a gold layer those nanostructured fibers exhibit interesting plasmonic properties. We wish to take advantage of those optical properties to perform remote biological analysis. For this, we plan to design an array of different biological probes at the nanotextured bundle end face to confer specific bio-sensing properties. The so formed device will be used “in vivo” for biological targets multiplexed specific detection in a remote mode.
This project is highly interdisciplinary as it combines skills at the forefront of optics, electrochemistry, surface chemistry, biochemistry, nano- and micro-technologies applied to the biological field. The work will be done by four complementary research groups (ISM / NSYSA, SPRAM / CREAB, LAAS / NBS, IAB/ AICD). The main steps of the project are schematically presented below. The fibers bundles will be etched and metalized in ISM (Bordeaux). This group is specialized in the development of new analytical nanosystems for biological applications by combining mainly optical and electrochemical techniques at the nanometer scale. LAAS (Toulouse) will provide patterning tools for the localized biofunctionalization of fibers surfaces by means of e.g. micromachined cantilevers thanks to their skills in micro- and nanotechnologies. SPRAM (Grenoble) will develop a dedicated optical setup in order to characterize the sensitivity of the designed probe. SPRAM has an expertise in the field of surface plasmon resonance (SPR) bio-chip functionalization and instrumentation development. IAB (Grenoble) studies the immune system and its digressions in chronic pathologies associated to inflammations. They will provide the required knowledge to qualify the bundles in model tissue before validating the tool efficiency for “in vivo” multiplexed molecular detection in an endoscopic and minimally-invasive way in a mouse model.

Project coordination

Loic Leroy (Structures et Propriétés d'Architectures Moléculaires)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

IAB - Inserm U823 Institut Albert Bonniot CR UJF/Inserm U823
CNRS/LAAS Laboratoire d'Analyse et d'Architecture des Systèmes
ISM Institut des Sciences Moléculaires
SPrAM Structures et Propriétés d'Architectures Moléculaires

Help of the ANR 397,094 euros
Beginning and duration of the scientific project: September 2015 - 36 Months

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