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Receptor-based multipotent artzymes – REBAR

Receptor Based Artificial Enzymes

The importance of catalysis for the development of sustainable chemistry and the resulting societal impact is unambiguously recognized. This project proposed portrays novel ecofriendly biocatalysts, namely “artzymes”, which are formed using the “Trojan horse strategy” by combining the a membrane receptor with catalytically active organometallic complexes. Such a combination constitute a novel approach featuring the major advantage of creating multipotent artificial enzymes.

Preparation and evaluation of receptor based artzymes.

The initial goal is to create an artzyme capable of catalyzing a desired reaction by anchoring a metal complex into the A2A Adenosine Receptor (AR).<br />After creating and evaluating the catalytic potential of receptor-­based artzymes the subsequent aim is to achieve multipotency by inciting the receptor to exchange organometallic partners.

1) Preparation of catalytic entities and evaluation of their catalytic potential.
2) Preparation/obtention of the A2A AR.
3) Preparation of the artzymes by incubating desired catalytic entity with the A2A AR & assays of catalysis with the formed artzymes.
4) assays of multipotency.
5) Dissemination of results.

One rhodium based catalytic entity was prepared.
Two copper based catalytic entities are in the final stage of preparation.

1) Preparation of artzymes using these catalytic entities and the commercially available A2A adenosine receptor.
2) Catalysis of hydrogenation reactions with the rhodium based artzyme and Diels Alder cyclisation reactions with the copper based artzymes.
3) Evaluation of multipotency.

Results are not published yet.

The importance of catalysis for the development of sustainable chemistry and the resulting societal impact is unambiguously recognized. The project proposed herein portrays novel eco-friendly biocatalysts, namely “artzymes”, which are formed using the “Trojan horse strategy” by combining the adenosine A2A receptor with catalytically active organometallic complexes. The catalytic activity of the artzymes will be evaluated in Diels-Alder cyclizations and in the selective hydrogenation of double bonds. An agonist or an antagonist of the A2A adenosine receptor will be linked to a copper complex or a rhodium complex, respectively, and subsequently combined with the receptor. Such a combination will take place either on the surface of cells expressing the receptor, leading to the first example of in cellulo catalysis by artificial enzymes, or on membrane debris containing the receptor. Such use of a receptor is a novel approach featuring the major advantage of creating multipotent artificial enzymes, which are capable of catalyzing cascades of different reactions. This will proof the concept of mutlicatalysis in a single-pot wherein a substrate is converted into desired products by a cascade of different reactions.

Project coordination

Wadih Ghattas (Laboratoire de Chimie Bioorganique et Bioinorganique) – wadih.ghattas@u-psud.fr

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

LCBM Laboratoire de Chimie Bioorganique et Bioinorganique

Help of the ANR 562,640 euros
Beginning and duration of the scientific project: October 2012 - 36 Months

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