Blanc SVSE 7 - Blanc - SVSE 7 - Biodiversité, évolution, écologie et agronomie

EVOlutionary and FUNctional analyses of the contribution of the MTP1 gene family to the acquisition of zinc tolerance in Arabidopsis halleri. – EVOFUN

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The work is still in progress. No data has been published yet.

Submission summary

This proposal aims at uncovering the contribution of different members of a gene family to the acquisition of the zinc tolerance trait in the wild zinc tolerant and hyperaccumulating plant species Arabidopsis halleri. As such, the presented proposal questions the fundamental process of adaptive evolution of gene function with a particular emphasis on the role of gene duplication. The originality of this proposal is that it combines evolutionary and functional approaches. Besides its fundamental interest, this proposal should have a major interest for phytoremediation.
A. halleri is assumed to have diverged less than 15 Million years ago from its closest non-tolerant relatives Arabidopsis lyrata and Arabidopsis thaliana. The acquisition of the zinc tolerance character in A. halleri was proposed to mainly rely on the duplication of genes controlling zinc homeostasis. The Metal Tolerance Protein 1 gene (MTP1) is one of these genes. We previously demonstrated that the MTP1 gene is pentaplicated in A. halleri, whereas the non-tolerant A. thaliana and A. lyrata were shown to possess only a single copy. The fact that the pentaplication of MTP1 would contribute to the acquisition of zinc tolerance in A. halleri is however strongly challenged. Actually our preliminary work suggests that members of the AhMTP1 family could have experienced different evolutionary fates In particular, some of them may not be involved in the zinc tolerance genetic determinism.
This proposal has the ambition to determine the actual evolutionary fate of each AhMTP1 paralog considering their contribution to zinc tolerance. Therefore, this proposal will combine evolutionary and functional approaches. Molecular evolutionary approaches will determine the pattern of genetic polymorphism within and among AhMTP1 copies in a large set of A. halleri natural populations from different geographic origins, including metallicolous and non-metallicolous populations. In parallel, a functional study of the AhMTP1 gene family will be performed to assess the actual contribution of each of the AhMTP1 paralogs to zinc tolerance. The original merging of evolutionary and functional approaches is expected to offer a unique opportunity to clearly identify which AhMTP1 played a critical role in the evolution of the zinc tolerance in A. halleri. Completion of this work will also deliver the precise alleles and functional nucleotide polymorphisms underlying adaptive polymorphisms for this trait. Altogether, our results will extend our current knowledge on critical gene function for zinc tolerance. This work should also provide information beneficial to environmental applications such as the engineering of better strategies for phytoremediation.

Project coordinator

Monsieur Pierre BERTHOMIEU (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - DELEGATION REGIONALE LANGUEDOC-ROUSSILLON) – berthomieu@supagro.inra.fr

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

UMR BPMP CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - DELEGATION REGIONALE LANGUEDOC-ROUSSILLON
GEPV CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - DELEGATION REGIONALE NORD-PAS-DE-CALAIS ET PICARDIE

Help of the ANR 280,000 euros
Beginning and duration of the scientific project: April 2012 - 36 Months

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