Bio-E - Bioénergies

Engineering the best-characterized cyanobacterium Synechocystis for a better photoproduction of hydrogen – EngineeringH2Cyano

Submission summary

Photoautotrophic production of hydrogen by cyanobacteria is of great public interest in promising a clean renewable energy carrier from nature's most plentiful resources, solar energy and water. Previous investigations in this frame led to protocols that generate only a slight and transient increase in H2 evolution, at the expense of reduction in cell growth. Therefore, as an important milestone in the future engineering of robust cyanobacteria for industrial-level photoproduction of hydrogen we propose to (i) use our long-term (25 years) experience in cyanobacterial genetics to construct robust mutants overproducing the hydrogen-evolving machine with no reduction of cell fitness; (ii) thoroughly analyze these mutants to characterize the metabolic adaptation to high-level production of H2; and (iii) use a high throughput protein-protein interaction assay for the identification of novel key players in H2 evolution. For this purpose, we will use the best-characterized cyanobacterium Synechocystis PCC6803 that (i) grows well in diverse environmental conditions; (ii) possesses a small genome fully sequenced (about 4.0 Mb) easily manipulable with the replicating plasmids we made for constitutive or regulated overproduction of proteins and analysis of their sub-cellular localization.

Project coordination

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

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Beginning and duration of the scientific project: - 0 Months

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