PCV - Programme interdiciplinaire en physique et chimie du vivant

Far field, 3D, real time optical nanoscopy optimized for cellular imaging – Resolution-XXL

Submission summary

The LPEM has developed for two years a structured illumination microscope that can produce 3D super resolved fluorescent images. The instrument, based on a commercial microscope is actually in a test phase. Lateral and axial resolution gains have been demonstrated independently on model samples, like fluorescent beads. The goal of our project is to obtain simultaneously in cells a lateral resolution better than 100 nanometers and an axial resolution better than 50 nanometers. This set up should be able to obtain 3D optical sections of complex biological objects faster than one image per second. The project has two parts: an instrumental part which will optimize the existing set up for the imaging of biological samples; and a mathematical part that will develop various data analysis and image processing algorithms necessary for the real time 3D reconstruction of super resolved optical sections. The AIQ lab will be in charge of the latter part of the project as it has specialized in image processing and analysis for biological microscopy. It has proposed a number of Gaussian approximation models for the PSFs of wide field and confocal microscopes, and ultra sensitive methods to localize spots in noisy biological images.

Project coordination

Vincent LORIETTE (Organisme de recherche)

The author of this summary is the project coordinator, who is responsible for the content of this summary. The ANR declines any responsibility as for its contents.

Partner

INSTITUT PASTEUR

Help of the ANR 320,000 euros
Beginning and duration of the scientific project: - 24 Months

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